(A) Expression pattern of two NDC80 mRNA isoforms during starvation and early meiosis. Diploid control cells (FW4644) or cells harboring the CUP1 promoter fused with IME1 and IME4 (pCUP-IME1/pCUP-IME…
(A) The same northern blot and western blot of NDC80 expression during a meiotic time course as described in Figure 1A, except that CIT1 and ribosomal RNA expression levels are also displayed. (B) …
(A) NDC80luti transcription promotes histone H3 lysine 4 dimethylation (H3K4me2) in the NDC80ORF promoter. Wild-type (FW1902) and set1Δ (FW3033) cells harboring the pCUP-IME1/pCUP-IME4 were induced …
(A) Set2 and Set3 are required for repression of NDC80ORF expression during early meiosis. Control (FW1902), set2Δ (FW2929), set3Δ (FW2928) and set2Δset3Δ (FW1922) cells harboring pCUP-IME1/pCUP-IME4…
(A) Quantification of NDC80ORF and NDC80luti levels in the experiment described in Figure 3A. Signals are normalized to SCR1. To control for variation in overall signal between different northern …
(A) Schematic overview of the experimental set-up. (B) Cells repress NDC80luti and induce NDC80ORF when returned to a nutrient rich environment (YPD). Cells harboring ndt80Δ and pCUP-IME1/pCUP-IME4 …
(A) Quantification of NDC80luti during return to rich medium as described in Figure 4B. RNA was extracted, reverse transcribed, and NDC80luti mRNA levels were determined by quantitative PCR. Signals …
(A) Adjustable expression of NDC80luti using the LexA-lexO system. Spot assay of cells harboring 0, 1, 2, 3, or 8 lexO binding sites in the NDC80luti promoter (UB8374, UB8358, UB8362, UB8366, …
(A) NDC80luti levels in the presence of variable number of lexO sites in the NDC80luti promoter. Cells harboring 0, 1, 2, 3, or 8 lexO and LexA-ER-AD (UB8374, UB8358, UB8362, UB8366, and UB8370) …
During mitosis the NDC80ORF promoter has an open chromatin structure and NDC80ORF is transcribed. During meiotic S-phase and meiotic prophase, transcription of NDC80luti deposits Set1-dependent …
Table of yeast strains used in this study.
Table describing genotypes of the strains.
Table of oligo nucleotide sequences used in this study.
Table describing oligo nucleotide sequences used for quantitative PCR and for preparing the northern blot probes.