Fish larval recruitment to reefs is a thyroid hormone-mediated metamorphosis sensitive to the pesticide chlorpyrifos
- Université de Lyon, Université Claude Bernard Lyon 1, UMR CNRS 5242, Ecole Normale Supérieure de Lyon, France
- PSL Research University, French Polynesia
- UMR CNRS 7232, Université Pierre et Marie Curie Paris, France
- Laboratoire d'Excellence CORAIL, French Polynesia
Figures
Figure 1 with 1 supplement
Changes in Acanthrurus triostegus during larval recruitment.
Error bars represent standard errors. *p-value<0.05; **p-value<0.01; ***p-value<0.001. (A) Schematization of the reef colonization by a larva (left) turning into a juvenile (right). The background …
-
Figure 1—source data 1
Acanthurus triostegus weight at different developmental stages.
- https://doi.org/10.7554/eLife.27595.005
-
Figure 1—source data 2
Acanthurus triostegus intestine length at different developmental stages.
- https://doi.org/10.7554/eLife.27595.006
Figure 1—figure supplement 1
Changes in Acanthurus triostegus intestine and teeth during larval recruitment.
(A–O) Complete guts histology of Acanthurus triostegus after reef colonization. First column: anterior part of the intestine (first cm), middle column: medium part (variable), last column: posterior …
Figure 2 with 3 supplements
Thyroid hormone signaling during Acanthurus triostegus metamorphosis.
Error bars represent standard errors. *p-value<0.05; **p-value<0.01; ***p-value<0.001. (A) T4 and (B) T3 dosage: Y-axis: T4 and T3 levels in pg.g−1; X-axis: sampling point. n = 3 for 5 to 10 km …
-
Figure 2—source data 1
Acanthurus triostegus T4 levels in larvae and juveniles.
- https://doi.org/10.7554/eLife.27595.011
-
Figure 2—source data 2
Acanthurus triostegus T3 levels in larvae and juveniles.
- https://doi.org/10.7554/eLife.27595.012
-
Figure 2—source data 3
TR and Klf9 expression levels in Acanthurus triostegus at different developmental stages (qPCR).
- https://doi.org/10.7554/eLife.27595.013
-
Figure 2—source data 4
Transactivation assay of Acanthurus triostegus TR for several thyroid hormone derivatives.
- https://doi.org/10.7554/eLife.27595.014
-
Figure 2—source data 5
Transactivation assay of Acanthurus triostegus TR for the competition of T3 and NH3.
- https://doi.org/10.7554/eLife.27595.015
-
Figure 2—source data 6
List of primers used in this study.
- https://doi.org/10.7554/eLife.27595.016
-
Figure 2—source data 7
List of TR used for phylogenetic reconstruction.
- https://doi.org/10.7554/eLife.27595.017
Figure 2—figure supplement 1
Characterization of Acanthurus triostegus thyroid hormone receptors.
(A) Schematic representations of TRα-A, TRα-B and TRβ from upper to lower rows respectively. The amino acid numbering of the DBD (light gray) and LBD (dark grey) appear above each TR. The nine amino …
Figure 2—figure supplement 2
Primers used in this study.
(A) Degenerated primers used for the cloning of the A. triostegus genes in this study. (B) qPCR primers used in this study. (C) Primers used for 18S mass sequencing. The overlapping nucleotides …
Figure 2—figure supplement 3
List of thyroid hormone receptors sequences used for the phylogeny.
Species, genes, databases and accession numbers are indicated. See Figure 2—source data 7.
Figure 3 with 2 supplements
Impact of the environment and thyroid hormone signaling on Acanthurus triostegus metamorphic processes.
Error bars represent standard errors. *p-value<0.05; **p-value<0.01; ***p-value<0.001. (A) Upper panel: schematization of the external slope experiment with control fish, in the lagoon (grey …
-
Figure 3—source data 1
T4 levels in external slope relocated Acanthus triostegus.
- https://doi.org/10.7554/eLife.27595.021
-
Figure 3—source data 2
T3 levels in external slope relocated Acanthus triostegus.
- https://doi.org/10.7554/eLife.27595.022
-
Figure 3—source data 3
TR and Klf9 expression levels in external slope relocated Acanthurus triostegus (qPCR).
- https://doi.org/10.7554/eLife.27595.023
-
Figure 3—source data 4
Acanthurus triostegus intestine length under pharmacological treatments or relocation.
- https://doi.org/10.7554/eLife.27595.024
-
Figure 3—source data 5
Acanthurus triostegus grazing activity under pharmacological treatments or relocation.
- https://doi.org/10.7554/eLife.27595.025
Figure 3—figure supplement 1
Thyroid hormone signaling and environment impact on the dentition and guts histology of Acanthurus triostegus at metamorphosis.
First column: X-Ray microtomography of larva, dentition A, B or C are highlighted respectively in red, blue or green. Scale bars indicate 0.5 mm. These pictures are representative of n = 3 fish …
Figure 3—figure supplement 2
Pigmentation patterns and thyroid hormone signaling in Acanthurus triostegus at day 2 (d2) and day (d5) during larval recruitment.
These pictures are representative of n > 10 treatment technical replicates performed on n > 100 fish (biological replicates) per treatment and per developmental stage.
Figure 4
Thyroid hormone levels in distant coral reef fish at larval recruitment.
Error bars represent standard errors. *p-value<0.05; **p-value<0.01; ***p-value<0.001. TH levels of: Rhinecanthus aculeatus (upper), Chromis viridis (middle up), Chaetodon lunula (middle low) and Ost…
-
Figure 4—source data 1
T4 and T3 levels of various coral reef fishes at different development stages.
- https://doi.org/10.7554/eLife.27595.027
Figure 5 with 1 supplement
Influence of chlorpyrifos exposure on Acanthurus triostegus thyroid hormone signaling, intestine length, grazing activity, and algal removal efficiency.
Error bars represent standard errors. *p-value<0.05; **p-value<0.01; ***p-value<0.001. (A) T3 level in pg.g−1 of day 2 (d2) and day 5 (d5) fish exposed to different conditions: solvent control (C, …
-
Figure 5—source data 1
T3 levels in Acanthurus triostegus under different chlorpyrifos exposure conditions.
- https://doi.org/10.7554/eLife.27595.030
-
Figure 5—source data 2
Intestine length of Acanthurus triostegus under different chlorpyrifos exposure conditions.
- https://doi.org/10.7554/eLife.27595.031
-
Figure 5—source data 3
Grazing activity of Acanthurus triostegus under different chlorpyrifos exposure conditions.
- https://doi.org/10.7554/eLife.27595.032
-
Figure 5—source data 4
Quantity of grazed algae by Acanthurus triostegus under different chlorpyrifos exposure conditions.
- https://doi.org/10.7554/eLife.27595.033
-
Figure 5—source data 5
T4 levels in Acanthurus triostegus under different chlorpyrifos exposure conditions.
- https://doi.org/10.7554/eLife.27595.034
Figure 5—figure supplement 1
Pigmentation patterns and chlorpyrifos exposure in A. triostegus at metamorphosis.
(A) T4 level in pg.g−1 of day 2 (d2) and day 5 (d5) fish exposed to different conditions: solvent control (C, acetone, white bars), or exposed to CPF at 1 µg.l−1 (1, light yellow bars), 5 µg.l−1 (5, …
Figure 6
Model of Acanthurus triostegus metamorphosis and disruption by chlorpyrifos.
(A) Schematic representation of coral reef fish larval recruitment. TH level is plotted on the Y axis and development on the X axis. The onset of metamorphosis is in the open ocean and environmental …
Additional files
-
Transparent reporting form
- https://doi.org/10.7554/eLife.27595.036
