Representative FACS plots of (A) FoxP3+ Tregs, gated on CD45+CD3e+CD4+ T cells, (B) CD4+ Th effectors following PMA and ionomycin stimulation for 4.5 hr, gated on CD45+CD3e+CD4+ T cells, (C) Type three innate lymphoid cells (ILC3), gated on CD3e-CD45loThy1.2hi cells, and (D) DCs, gated on CD45+CD11c+ cells in the LP of 2 and 3–4 week old Il10rb-/- and littermate Il10rb+/- controls. Comparison of the frequencies and absolute numbers per colon of (E) FoxP3+ Tregs, (F) CD4+ Th effectors, (G) ILC3s, and (H) DCs is shown for Il10rb-/- vs control Il10rb+/- mice at 2 and 3–4 weeks of age. Results are pooled from 2 to 3 litters leading to 7–11 mice in each group. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, Mann-Whitney U test. Data represents Median with IQR.