(A) The Zfp36-V5 mice were born in the predicted Mendelian ratio from heterozygous matings. The Zfp36-V5 mice from heterozygous matings were genotyped at 3–4 weeks old. (B) BMDMs from the Zfp36-V5 mice have the normal inflammatory response. BMDMs from the Zfp36-V5 mice and the wild-type mice were treated with 100 ng/ml LPS, and the cells were harvested at 0, 1, 2, 4, 6, 8 hr post LPS treatment. The levels of the indicated mRNAs were quantified by qRT-PCR, and 18S rRNA was used for normalization. The results represent the means (±SD) of three independent experiments. (C) Zfp36 can be unambiguously detected in the Zfp36-V5 mouse. BMDMs from the Zfp36-V5 mice were treated with 100 ng/ml LPS, and cells were harvested at 0, 2, 4, 6 hr post LPS treatment for SDS-PAGE and Western blot using the indicated antibodies.