Prolyl dihydroxylation of unassembled uS12/Rps23 regulates fungal hypoxic adaptation
- Johns Hopkins University School of Medicine, United States
Abstract
The prolyl-3,4-dihydroxylase Ofd1 and nuclear import adaptor Nro1 regulate the hypoxic response in fission yeast by controlling activity of the sterol regulatory element-binding protein transcription factor Sre1. Here, we identify an extra-ribosomal function for uS12/Rps23 central to this regulatory system. Nro1 binds Rps23, and Ofd1 dihydroxylates Rps23 P62 in complex with Nro1. Concurrently, Nro1 imports Rps23 into the nucleus for assembly into 40S ribosomes. Low oxygen inhibits Ofd1 hydroxylase activity and stabilizes the Ofd1-Rps23-Nro1 complex, thereby sequestering Ofd1 from binding Sre1, which is then free to activate hypoxic gene expression. In vitro studies demonstrate that Ofd1 directly binds Rps23, Nro1, and Sre1 through a consensus binding sequence. Interestingly, Rps23 expression modulates Sre1 activity by changing the Rps23 substrate pool available to Ofd1. To date, oxygen is the only known signal to Sre1, but additional nutrient signals may tune the hypoxic response through control of unassembled Rps23 or Ofd1 activity.
Article and author information
Author details
Funding
National Institutes of Health (HL077588)
- Peter Espenshade
American Heart Association (Predoctoral Fellowship 13PRE15820017)
- Sara J Clasen
American Heart Association (Grant in Aid 13GRNT16400001)
- Peter Espenshade
National Institutes of Health (T32 GM007445)
- Sara J Clasen
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2017, Clasen et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Prolyl dihydroxylation of unassembled uS12/Rps23 regulates fungal hypoxic adaptation
eLife 6:e28563.
https://doi.org/10.7554/eLife.28563
