(A) Trypsin genes abundance after dsRNA-mediated gene silencing, (AAEL010196 (T196), AAEL013707 (T707), AAEL013714 (T714), AAEL013715 (T715). (B–F) DENV infection intensity of trypsin genes-silenced mosquitoes are compared to GFP dsRNA-treated control mosquitoes (B) T196 (Control, N = 54; T196, N = 54), (C) T707 (Control, N = 71; T707, N = 69), (D) T714 (Control, N = 71; T714, N = 62), (E) T715 (Control, N = 71; T715, N = 72), (F) Simultaneous silencing of all trypsins (Tmix) (Control, N = 71; Tmix, N = 71). The line indicates the mean, each dot represents the log10 PFU after 7 dpi in individual midguts from four independent biological experiments, except T196 which represents three independent experiments. Upper right boxes show the prevalence of infected mosquitoes, error bars represent the 95% confidence interval. *p<0.05, ***p<0.001.