(A) Domain structures of Pan1, End3 and Sla1. EH, Eps15 homology; CC, coiled-coil domain; PRD, proline rich domain; SH3, Src homology 3; SHD1, Sla1 homology domain 1; SHD2, Sla1 homology domain 2; CBM, clathrin-binding motif; SR repeats, LxxQxTG repeats. (B) Examining cell growth of indicated yeast strains by spotting serial dilutions of liquid cultures on plate at 30°C. (C) Immunoblot analysis of whole-cell extracts from indicated yeast cells. PGK (phosphoglycerate kinase) serves as a loading control. (D) Single frame from a movie in which SLA1-GFP PAN1-mCherry cells and sla1W41AW108A-GFP cells were simultaneously imaged in the GFP channel. PAN1-mCherry was used to identify wild-type cells. (E) Single frame from a movie in which PAN1-GFP SLA1-mCherry cells and pan1∆PRD-GFP cells were simultaneously imaged in the GFP channel. SLA1-mCherry was used to identify wild-type cells. The scale bars on cell pictures are 2 µm.