Two photon ablation on neuromasts of the double transgenic Tg(K15:H2B-RFP), Tg(Eya1:EGFP) at 12 dpf. (A–B’). Ablations were done to remove most cells in the neuromasts, sparing a few K15+ cells (B’). The same neuromast shown 40 hr post-ablation reveals a small cluster of RFP +cells that have coalesced around the site of injury without any apparent differentiation (C–C’’). Six days post-injury all cell types within the neural lineage have been reconstituted (D–D’’). Mantle and support cells can be observed in K15:H2B-RFP (D, D’’), while hair cells and internal support cells are evident in the Eya1:EGFP (D’, D’’). (N = 18 neuromasts in four larvae for Tg(K15:H2B-RFP), Tg(Eya1:EGFP)), N = 8 neuromasts in four larvae for Tg(K15:H2B-RFP)(neuromK8:H2B-EGFP)) Scalebars are 100 µm for trunks (A, A’) and 10 µm for neuromasts (B–D’’).