Magnetic eye tracking in mice
- Stanford University, United States
Figures
Figure 1 with 1 supplement
Systematic testing of magnet-sensor placement.
(A) Output of the magnetic sensor when a 0.75 × 2 mm cylinder magnet was rotated by a servo-controlled motor. Left, Schematic showing the relative position and orientation of the magnet and sensor, …
Figure 1—figure supplement 1
Systematic testing of magnet-sensor placement for a disc magnet.
(A) Output of the magnetic sensor when a 1.5 × 0.5 mm disc magnet was rotated by a servo-controlled motor. Left, schematic of magnet-sensor orientation. Middle, output from each channel of the …
Figure 2
Magnetic eye tracking in mice.
(A) Schematic of the magnetic eye tracking system, illustrating the orientation of the sensor relative to the magnet and eye. The magnet was implanted beneath the conjunctiva on the temporal side of …
Figure 3
Dual-angle video-oculography.
(A) Schematic of the eye as viewed from above, illustrating the dual-angle video-oculography technique. Two cameras were affixed to a platform, with their axes at an angle of 40° relative to each …
Figure 4
Calibration of magnetic eye tracking using video-oculography.
(A) Simultaneously recorded magnetic sensor output (Ch1 dark blue, Ch2 light blue) and video-derived eye position (black) from one example mouse, measured during vestibular stimulation in the light. …
Figure 5
Comparison of magnetic eye tracking with the eye coil technique.
(A) Correlation coefficient between the video and the eye coil data (gray; symbols represent individual mice, n = 9) and between the video and magnetic eye tracking data (blue, n = 8 mice) (p=0.937; …
Figure 6 with 2 supplements
The vestibulo-ocular reflex (VOR) before and after magnet implantation.
The gain (left) and phase (right) of the eye movement responses to vestibular stimuli, before (black) and six days after (blue) implantation of the magnet and sensor, measured using …
Figure 6—figure supplement 1
Eye movements driven by combined vestibular and visual input, before and after magnet implantation.
The gain (left) and phase (right) of the eye movement responses to the combined vestibular and visual input provided by head rotations in an illuminated visual surround was measured using …
Figure 6—figure supplement 2
The optokinetic response (OKR) measured with magnetic eye tracking.
The gain (left) and phase (right) of the OKR elicited by rotation of a random checkerboard patterned drum around the mouse, measured using the magnetic eye tracking system (n = 6 mice).
Figure 7
Eye movements measured in freely moving mice.
(A) Example of bilateral eye movements in one mouse when it was head-fixed (top) compared to freely moving (bottom). Light purple, body still; dark purple, body actively moving, as measured using …
Additional files
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Transparent reporting form
- https://doi.org/10.7554/eLife.29222.012
