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Revealing the distribution of transmembrane currents along the dendritic tree of a neuron from extracellular recordings

  1. Dorottya Cserpán
  2. Domokos Meszéna
  3. Lucia Wittner
  4. Kinga Tóth
  5. István Ulbert
  6. Zoltán Somogyvári
  7. Daniel K Wojcik  Is a corresponding author
  1. Hungarian Academy of Sciences, Hungary
  2. Nencki Institute of Experimental Biology, Poland
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Cite this article as: eLife 2017;6:e29384 doi: 10.7554/eLife.29384

Abstract

Revealing the current source distribution along the neuronal membrane is a key step on the way to understanding neural computations, however, the experimental and theoretical tools to achieve sufficient spatiotemporal resolution for the estimation remain to be established. Here we address this problem using extracellularly recorded potentials with arbitrarily distributed electrodes for a neuron of known morphology. We use simulations of models with varying complexity to validate the proposed method and to give recommendations for experimental applications. The method is applied to in vitro data from rat hippocampus.

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Author details

  1. Dorottya Cserpán

    Wigner Research Centre for Physics, Hungarian Academy of Sciences, Budapest, Hungary
    Competing interests
    The authors declare that no competing interests exist.

  2. Domokos Meszéna

    Institute of Cognitive Neuroscience and Psychology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary
    Competing interests
    The authors declare that no competing interests exist.

  3. Lucia Wittner

    Institute of Cognitive Neuroscience and Psychology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary
    Competing interests
    The authors declare that no competing interests exist.

  4. Kinga Tóth

    Institute of Cognitive Neuroscience and Psychology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary
    Competing interests
    The authors declare that no competing interests exist.

  5. István Ulbert

    Institute of Cognitive Neuroscience and Psychology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary
    Competing interests
    The authors declare that no competing interests exist.

  6. Zoltán Somogyvári

    Wigner Research Centre for Physics, Hungarian Academy of Sciences, Budapest, Hungary
    Competing interests
    The authors declare that no competing interests exist.

  7. Daniel K Wojcik

    Department of Neurophysiology, Nencki Institute of Experimental Biology, Warsaw, Poland
    For correspondence
    d.wojcik@nencki.gov.pl
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-0812-9872

Funding

Ministerstwo Nauki i Szkolnictwa Wyższego (Grant 2729/7.PR/2013/2)

  • Daniel K Wojcik

Nemzeti Kutatási, Fejlesztesi és Innovacios Hivatal (Grant K 113147)

  • Zoltán Somogyvári

Nemzeti Agykutatasi Program (Grant KTIA NAP 13-1-2013-0001)

  • István Ulbert

Nemzeti Kutatasi, Fejilesztesi es Innovacios Hivatal (Grant NN 118902)

  • Zoltán Somogyvári

Nemzeti Agykutatasi Program (Grant KTIA-13-NAP-A-IV/1 2 3 4 6)

  • István Ulbert

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Ethics

Animal experimentation: The in vitro experiment was performed according to the EC Council Directive of November 24, 1986 (86/89/EEC) and all procedures were reviewed and approved by the local ethical committee and the Hungarian Central Government Office (license number: PEI/001/695-9/2015).

Reviewing Editor

  1. Frances K Skinner, Krembil Research Institute, University Health Network, Canada

Publication history

  1. Received: June 7, 2017
  2. Accepted: November 16, 2017
  3. Accepted Manuscript published: November 17, 2017 (version 1)
  4. Version of Record published: December 5, 2017 (version 2)
  5. Version of Record updated: December 5, 2017 (version 3)
  6. Version of Record updated: February 20, 2018 (version 4)

Copyright

© 2017, Cserpán et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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