A Hotelling T2 test was performed to evaluate the difference in mean shape between mutant and wildtype groups; p-value, test statistic, and sample size (N) are shown. The Procrustes distances between mutant and wild type mean shapes are also indicated. *The comparison between heterozygous and homozygous mice for the Mitfmi-vga9 mutation is also shown. (B) Missense variants found in the gene Mitf of wild mice. Nine populations of wild mice were screened for SNPs causing coding changes in Mitf: Mus musculus musculus from Kazakhstan, Check Republic, and Afganistan; M. m. domesticus from Iran, Heligoland, France, and Germany; Mus castaneus; and Mus spretus. Data available in the UCSC browser → MyData - > Public Sessions - > wildmouse (Harr et al., 2016). In addition, eight hybrids between M.m. musculus and M.m. domesticus from the German hybrid zone (Turner, Tautz and Harr unpublished data), the same population used in this study, were also screened for coding changes. Reference and variant alleles are shown. nVar = number of chromosomes with the variant allele, n = number of chromosomes per population.