The zinc-finger transcription factor Hindsight regulates ovulation competency of Drosophila follicles

  1. Lylah D Deady
  2. Wei Li
  3. Jianjun Sun  Is a corresponding author
  1. University of Connecticut, United States

Abstract

Follicle rupture, the final step in ovulation, utilizes conserved molecular mechanisms including matrix metalloproteinases (Mmps), steroid signaling, and adrenergic signaling. It is still unknown how follicles become competent for follicle rupture/ovulation. Here, we identify a zinc-finger transcription factor Hindsight (Hnt) as the first transcription factor regulating follicle's competency for ovulation in Drosophila. Hnt is not expressed in immature stage-13 follicle cells but is upregulated in mature stage-14 follicle cells, which is essential for follicle rupture/ovulation. Hnt upregulates Mmp2 expression in posterior follicle cells (essential for the breakdown of the follicle wall) and Oamb expression in all follicle cells (the receptor for receiving adrenergic signaling and inducing Mmp2 activation). Hnt's role in regulating Mmp2 and Oamb can be replaced by its human homolog Ras-responsive element-binding protein 1 (RREB-1). Our data suggest that Hnt/RREB-1 plays conserved role in regulating follicle maturation and competency for ovulation.

Article and author information

Author details

  1. Lylah D Deady

    Department of Physiology and Neurobiology, University of Connecticut, Storrs, United States
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-9316-1326
  2. Wei Li

    Department of Physiology and Neurobiology, University of Connecticut, Storrs, United States
    Competing interests
    The authors declare that no competing interests exist.
  3. Jianjun Sun

    Department of Physiology and Neurobiology, University of Connecticut, Storrs, United States
    For correspondence
    jianjun.sun@uconn.edu
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-6015-738X

Funding

National Institutes of Health (R01-HD086175)

  • Jianjun Sun

Bill and Melinda Gates Foundation (OPP1160858)

  • Jianjun Sun

University of Connecticut (Startup)

  • Jianjun Sun

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Yukiko M Yamashita, University of Michigan, Ann Arbor, HHMI, United States

Publication history

  1. Received: June 23, 2017
  2. Accepted: December 18, 2017
  3. Accepted Manuscript published: December 19, 2017 (version 1)
  4. Version of Record published: January 15, 2018 (version 2)
  5. Version of Record updated: June 21, 2018 (version 3)

Copyright

© 2017, Deady et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Lylah D Deady
  2. Wei Li
  3. Jianjun Sun
(2017)
The zinc-finger transcription factor Hindsight regulates ovulation competency of Drosophila follicles
eLife 6:e29887.
https://doi.org/10.7554/eLife.29887

Further reading

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