P493-6 cells were grown in the presence of tetracycline (Tet) for 72 hr and switched into Tet-free growth medium to induce c-Myc expression. Cells were cultured in two separate lots of serum. (A) Representative Western blot using an anti-c-Myc antibody (top panels) or an anti-ß-Actin antibody (bottom panel). Two exposures of the anti-c-Myc antibody are presented to facilitate detection of c-Myc. (B) Quantification of total RNA levels (ng of total RNA per 1,000 cells) for cells at 0, 1, and 24 hr after release from Tet. Means reported and error bars represent s.e.m. from three independent biological repeats. For serum lot one, one-way ANOVA on total RNA levels of all groups; F(2, 6)=1.25, p=0.353. Planned contrast between 0 hr and 24 hr; t(6) = 1.02, p=0.347 with a priori alpha level = 0.05. For serum lot two, one-way ANOVA on total RNA levels of all groups; F(2, 6)=21.87, p=0.00176. Planned contrast between 0 hr and 24 hr; t(6) = 5.03, p=0.0024 with a priori alpha level = 0.05. Additional details for this experiment can be found at https://osf.io/tfd57/.