CtBP impedes JNK- and Upd/STAT-driven cell fate misspecifications in regenerating Drosophila imaginal discs
- University of California, Berkeley, United States
- Cited 1
- Views 1,637
- Annotations
Abstract
Regeneration following tissue damage often necessitates a mechanism for cellular re-programming, so that surviving cells can give rise to all cell types originally found in the damaged tissue. This process, if unchecked, can also generate cell types that are inappropriate for a given location. We conducted a screen for genes that negatively regulate the frequency of notum-to-wing transformations following genetic ablation and regeneration of the wing pouch, from which we identified mutations in the transcriptional co-repressor C-terminal Binding Protein (CtBP). When CtBP function is reduced, ablation of the pouch can activate the JNK/AP-1 and JAK/STAT pathways in the notum to destabilize cell fates. Ectopic expression of Wingless and Dilp8 precede the formation of the ectopic pouch, which is subsequently generated by recruitment of both anterior and posterior cells near the compartment boundary. Thus, CtBP stabilizes cell fates following damage by opposing the destabilizing effects of the JNK/AP-1 and JAK/STAT pathways.
Article and author information
Author details
Iswar K Hariharan
Funding
National Institute of General Medical Sciences (GM061672)
- Iswar K Hariharan
American Cancer Society (RP-16-238-06-COUN)
- Iswar K Hariharan
National Institute of General Medical Sciences (GM085576)
- Iswar K Hariharan
National Institute of General Medical Sciences (GM122490)
- Iswar K Hariharan
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Bruce Edgar, Reviewing Editor, University of Utah, United States
Publication history
- Received: July 13, 2017
- Accepted: January 19, 2018
- Accepted Manuscript published: January 26, 2018 (version 1)
- Version of Record published: February 22, 2018 (version 2)
Copyright
© 2018, Worley et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
Metrics
-
- 1,637
- Page views
-
- 317
- Downloads
-
- 1
- Citations
Article citation count generated by polling the highest count across the following sources: Crossref, Scopus, PubMed Central.
Download links
Downloads (link to download the article as PDF)
Download citations (links to download the citations from this article in formats compatible with various reference manager tools)
Open citations (links to open the citations from this article in various online reference manager services)
Further reading
-
- Chromosomes and Gene Expression
- Developmental Biology
- Stem Cells and Regenerative Medicine
-
- Stem Cells and Regenerative Medicine
