Systemic and local cues drive neural stem cell niche remodelling during neurogenesis in Drosophila
- University of Cambridge, United Kingdom
Figures
Figure 1 with 1 supplement
Individual Drosophila neural stem cells and their lineages are progressively enclosed in cortex glial chambers.
(A) Two waves of neurogenesis take place in the Drosophila CNS. NSCs divide asymmetrically during embryogenesis to form the larval nervous system. They then enter quiescence, from which they …
Figure 1—figure supplement 1
Organisation and formation of the cortex glial chamber.
(A) NSCs in late larval stages are in close proximity to the BBB and diverse of glial subtypes. (B) Activation of the PI3K/Akt pathway in NSCs triggers NSC reactivation. (C–D”) grh-GAL4 drives in …
Figure 2 with 1 supplement
Cortex glial remodelling depends on nutritional cues.
(A–A’) Cortex glia enclose each NSC and its lineage into an individual membrane chamber in control fed larvae. (A) Bottom and top panels, ventral and orthogonal views of a third instar VNC. Yellow …
Figure 2—figure supplement 1
NSC chamber formation is nutrition-dependent.
(A–A’) Blocking nutritional sensing and relay in the brain through gap junction RNAi knockdown in glia impairs NSC chamber formation. (A) 3D reconstruction of the thoracic section of one VNC, …
Figure 3 with 2 supplements
The insulin pathway is required in the cortex glia for autonomous chamber growth.
(A–F) Knockdown of insulin signalling specifically in the cortex glia impairs chamber formation. (A) 3D reconstruction of the thoracic section of one fed control VNC, ventral view. (A’) Top and …
Figure 3—figure supplement 1
The insulin receptor is required in the cortex glia for chamber formation.
Blocking InR function specifically in the cortex glia impairs chamber formation. (A) 3D reconstruction of the thoracic section of one fed control VNC, ventral view. (A’) Top and bottom panels, …
Figure 3—figure supplement 2
Cortex glial division is not required for NSC chamber formation.
(A–C) Knockdown of stg/cdc25 and overexpression of dacapo (dap) in cortex glia prevents cell division. Maximal projection of a VNC for control (A), stg knockdown (B) and dap overexpression (C) …
Figure 4 with 1 supplement
The insulin pathway is required in NSCs for non-autonomous cortex glial chamber remodelling.
(A–F) Knockdown of insulin signalling specifically in NSCs impairs chamber formation. (A) 3D reconstruction of the thoracic section of one fed control VNC, ventral view. (A’) Top and bottom panels, …
Figure 4—figure supplement 1
NSC reactivation is crucial for cortex glial chamber organisation.
(A–B’) Delaying NSC reactivation alters chamber formation. (A) 3D reconstruction of the thoracic section of one VNC of Δp60 overexpression, ventral view. (A’) Top and bottom panels, orthogonal and …
Figure 5 with 2 supplements
Nutrition-dependent formation of the cortex glial chamber promotes survival of newly-born neurons.
(A) Selective manipulations of the PI3K/Akt pathway identify both autonomous and non-autonomous signals required for chamber formation. (B–D) Neuronal apoptosis is increased when chamber formation …
Figure 5—figure supplement 1
Role and formation of the cortex glial chamber.
(A–B) Impairing cortex glial chamber formation has little impact on NSCs. (A) VNC NSC counts are statistically comparable in control and Δp60 expression in cortex glia. p=0.17. n (control) = 10 …
Figure 5—figure supplement 2
Impairing dynamin-dependent signalling in NSCs has limited impact on chamber formation.
Expressing the thermosensitive allele shibirets1 in NSCs does not prevent chamber formation. (A) 3D reconstruction of the thoracic section of one fed control VNC, ventral view. (A’) Top and bottom …
Additional files
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Transparent reporting form
- https://doi.org/10.7554/eLife.30413.014
