Projections of confocal micrographs of eye discs stained for Atg8a (A–E) or p62 (F–J) from wild-type controls (A,F), p35 mutant larvae (B,G) or p35 mutant larvae expressing AcnWT (C,H), AcnS437A (D,I) or the phosphomimetic AcnS437D (E,J) under control of the acnP promoter. Scale bar in A is 40 µm in A-J. (K) Quantification of Atg8a punctae from three eye discs each with genotypes as shown in A-E. (L) Quantification of p62 punctae from three eye discs each with genotypes as shown in F-J. Bar graphs show mean ±SD. ns, not significant; **p<0.01; ***p<0.001; ***p<0.0001; each compared to wild-type Acn control. (M) Survival curves of WT controls or p35 mutants, or p35 mutants expressing the indicated Acn proteins. The initial numbers of flies were 95 (WT), 121 (p35), 116 (p35, AcnWT), 111 (p35, AcnS437A), 90 (p35, AcnS437D). Log rank tests compared to WT control: ns, not significant; ****p<0.0001. Detailed genotypes are listed in Supplementary file 3.