The pleural exudate cells were isolated from naïve and infected BALB/c or C57BL/6 mice at day 11, 28, 35 and 50 pi with L. sigmodontis. Prior to flow cytometry staining the cell number was calculated using a Nexcelom cell counter. (A) Cell number was then back-calculate based on the percentage of total cells positive for the aqua live-dead stain. (B) B cells were identified as Lin+ (CD19.Ly6G,SigLecF,TCRβ) MHC II +, Side Scatter Area (SSC-A) small. (C) T cells (Lin+, MHC II-, SSC-Asmall). Granulocytes were gated Lin+, MHC II-, SSC-Alarge (D) Eosinophils were identified as Lin+,MHC II-, SSC-Alarge, F4/80+ (E) Neutrophils were identified as Lin+, MHC II-, SCC-Alarge, F4/80-, CD11b+.(F) MΦ were identified as live, Lin- (CD19, Ly6G, SiglecF, TCRβ) CSF1R+ CD11c-. Total cells, Macrophages, and B cells are the same as Figure 1 and shown here for comparison. Presented are the data from two separate time course experiments (day 11 and 28 and day 35 and 50), each of which is representative of three independent experiments with 6 mice/group/time point. *p<0.05, **p<0.01, ***p<0.0001, ****p<0.00001 as determined by a 2-way ANOVA comparing infected C57BL/6 with infected BALB/c mice at each time point. Error bars represent the mean ± SEM.