(A) qPCR analyses (biological triplicates) of H3K27me2/3 ChIP with strains containing 8 or 17 telomere repeats at the csr-1 locus, with WT strain as a control (means displayed; error bars show standard deviation; single and double asterisks represent p=0.01488 and p=0.00336 respectively). Level of H3K27me2/3 normalized to Telomere 1L. MWT = 3.05970, SDWT = 0.64784, 95% CIWT = [2.32656, 3.79264]; M8 = 9.08627, SD8 = 3.09022, 95% CI8 = [5.58937, 12.58303]; M17 = 52.78110, SD17 = 14.35716, 95% CI17 = [36.53461, 69.02719]. (B) H3K27me2/3 ChIP-seq of full WT LG I (pooled biological triplicates; black track). Black circle indicates the centromere, csr-1 position is indicated by vertical line, and arrows at chromosome ends indicate 5’ to 3’ polarity of telomere repeats. Expansion below shows the extent and shape of the H3K27me2/3 domain induced in the Δcsr-1::(TTAGGG)17 strain (single sample; green track) compared to WT (black track). Site of csr-1 replacement with telomere repeats indicated below ChIP-seq tracks. Genes are displayed as black bars.