Food accumulation, represented by the total fluorescence (top row), and durations of foragers in the nest (bottom row), as a function of time in the two experimental colonies that underwent colony state manipulation (see Methods). Solid black line represents the time of introducing new hungry ants to the system. The results of colony M2 are presented in Figure 4d–e in the main text, where a detailed explanation may be found in the caption. Note that the plots depicting durations in the nest here differ from that in Figure 4e, in that here durations are plotted by two separate groups: those of foragers which were active during the whole experiment (M1: n = 47,13,15,13,12,16,17,14 for bins 1–8, respectively; M2: n = 28,36,21,14,5,15,18,10,4, for bins 1–9, respectively) and those of foragers that began foraging after the manipulation (M1: n = 6,3,2, for bins 1–3, respectively; M2: n = 4,13,9,9,1, for bins 1–5, respectively). In Figure 4e, all were pooled together. The observation that foragers of both groups displayed similar patterns after the manipulation highlights the causality of the effect of colony state on forager behavior as opposed to time or the forager’s history.