(A) Representative western blot images of α-, β-, and DT-tubulin content in all three genotypes. (B–D) Eliminating Nox2 ROS production decreases absolute α-, β- and DT-tubulin content in dystrophic diaphragm muscle. (E) The relative amount of DT-/α-tubulin is not different between groups. (F) Representative images of diaphragm myofibers stained with α-tubulin. (G–I) The lack of Nox 2 ROS prevents microtubule disorganization and the increase in microtubule density seen in mdx muscle. p≤0.05 *Significant difference between groups in at least (A–E) nanimals = 6 and (F–I) nanimals = 3 and nfibers = 15.