gene (Homo sapiens) | STING; TMEM173 | NA | GENBANK:NM_198282 | GENBANK:MF622062 |
gene (Homo sapiens) | STING; TMEM173 | this study | | GENBANK:MF622062 |
gene (Pan troglodytes) | STING; TMEM173 | NA | GENBANK:XM_016953921 | |
gene (Pan paniscus) | STING; TMEM173 | this study | | GENBANK:MF616339 |
gene (Gorilla gorilla) | STING; TMEM173 | NA | GENBANK:XM_0040426 | |
gene (Pongo abelii) | STING; TMEM173 | NA | GENBANK:XM_002815952 | |
gene (Hylobates agilis) | STING; TMEM173 | this study | | GENBANK:MF616342 |
gene (Symphalangus syndactylus) | STING; TMEM173 | this study | | GENBANK:MF616343 |
gene (Nomascus leucogenys) | STING; TMEM173 | this study | | GENBANK:MF616344 |
gene (Hylobates lar) | STING; TMEM173 | this study | | GENBANK:MF616341 |
gene (Rhinopithecus roxellana) | STING; TMEM173 | NA | GENBANK:XM_010388119 | |
gene (Rhinopithecus bieti) | STING; TMEM173 | NA | GENBANK:XM_017895026 | |
gene (Trachypithecus francoisi) | STING; TMEM173 | this study | | GENBANK:MF616352 |
gene (Colobus guereza) | STING; TMEM173 | this study | | GENBANK:MF616351 |
gene (Chlorocebus sabaeus) | STING; TMEM173 | NA | GENBANK:XM_008014636 | |
gene (Cercopithecus wolfi) | STING; TMEM173 | this study | | GENBANK:MF616350 |
gene (Miopithecus talapoin) | STING; TMEM173 | this study | | GENBANK:MF616349 |
gene (Macaca nemestrina) | STING; TMEM173 | NA | GENBANK:XM_011716377 | |
gene (Macaca mulatta) | STING; TMEM173 | NA | GENBANK:XM_015141010 | |
gene (Macaca mulatta) | STING; TMEM173 | this study | | GENBANK:MF622060 |
gene (Macaca fascicularis) | STING; TMEM173 | this study | | GENBANK:MF616346 |
gene (Papio papio) | STING; TMEM173 | this study | | GENBANK:MF616348 |
gene (Lophocebus albigena) | STING; TMEM173 | this study | | GENBANK:MF616347 |
gene (Cercocebus atys) | STING; TMEM173 | NA | GENBANK:XM_012090448 | |
gene (Mandrillus leucophaeus) | STING; TMEM173 | NA | GENBANK:XM_011997224 | |
gene (Aloutta sara) | STING; TMEM173 | this study | | GENBANK:MF616355 |
gene (Callicebus cupreus) | STING; TMEM173 | this study | | GENBANK:MF616354 |
gene (Callithrix jacchus) | STING; TMEM173 | NA | GENBANK:XM_00898588 | |
gene (Callithrix jacchus) | STING; TMEM173 | this study | | GENBANK:MF622061 |
gene (Cebus capucinus imitator) | STING; TMEM173 | NA | GENBANK:XM_017536735 | |
gene (Samiri boliviensis) | STING; TMEM173 | NA | GENBANK:XM_003933913 | |
gene (Saimiri sciureus) | STING; TMEM173 | this study | | GENBANK:MF616353 |
gene (Mus musculus) | STING; TMEM173 | NA | GENBANK:NM_001289591 | |
gene (Sus scrofa) | STING; TMEM173 | NA | GENBANK:XP_005661761 | |
gene (Heterocephalus glaber) | | NA | GENBANK:JAO02071 | |
gene (Chinchilla lanigera) | | NA | GENBANK:XP_005382124 | |
gene (Neotoma lepida) | | NA | GENBANK:OBS58238 | |
gene (Dengue viurs 2) | NS2B3 | NA | GENBANK:M29095 | |
cell line (Homo sapiens) | 293T cells | ATCC | CRL-3216 | |
cell line (Homo sapiens) | A549 cells | ATCC | CCL-185 | |
antibody | Rat anti-HA-HRP (3F10) | Sigma | 11867423001 | |
antibody | Mouse anti-Flag (M2) | Sigma | F3165 | |
antibody | Rabbit anti-pIRF3 | abcam | ab76493 | |
antibody | Rabbit anti-IRF3 | Santa Cruz Biotech | sc-9082 | |
antibody | Rabbit anti-GAPDH | Cell Signaling | 14C10 | |
antibody | Rabbit anti-STING | abcam | ab92605 | |
antibody | Mouse anti-Actin (C4) | Santa Cruz Biotech | Sc47778 | |
recombinant DNA reagent | DENV2 NS2B3 WT (plasmid) | PMID: 1642612 | | Progenitors: DENV2 NGC (GENBANK:M29095), pCR3.1 |
recombinant DNA reagent | DENV2 NS2B3 S135A (plasmid) | PMID: 1642612 | | Progenitors: DENV2 NS2B3 WT pCR3.1 plasmid, SDM |
recombinant DNA reagent | DENV1 (Hawaii) cDNA | this paper | | Progenitors: World Reference Center for Emerging Viruses and Arboviruses (WRCEVA) Catalog number NR-4287 |
recombinant DNA reagent | DENV2 (New Guinea C) cDNA | this paper | | Progenitors: World Reference Center for Emerging Viruses and Arboviruses (WRCEVA) Catalog number NR-4288 |
recombinant DNA reagent | DENV3 (Philippines/ H87/1956) cDNA | this paper | | Progenitors: World Reference Center for Emerging Viruses and Arboviruses (WRCEVA) Catalog number NR-2771 |
recombinant DNA reagent | DENV4 (H241) cDNA | this paper | | Progenitors: World Reference Center for Emerging Viruses and Arboviruses (WRCEVA) Catalog number NR-4289 |
recombinant DNA reagent | DENV1 (Hawaii) NS2B3 WT (plasmid) | this paper | | Progenitors: DENV1 (Hawaii) cDNA, pCR3.1 |
recombinant DNA reagent | DENV2 (New Guinea C) NS2B3 WT (plasmid) | this paper | | Progenitors: DENV2 (New Guinea C) cDNA, pCR3.1 |
recombinant DNA reagent | DENV3 (Philippines/ H87/1956) NS2B3 WT (plasmid) | this paper | | Progenitors: DENV3 (Philippines/H87/1956) cDNA, pCR3.1 |
recombinant DNA reagent | DENV4 (H241) NS2B3 WT (plasmid) | this paper | | Progenitors: DENV4 (H241) cDNA, pCR3.1 |
recombinant DNA reagent | Sylvatic (DakAr-141069) Dengue NS2B3 Protease (WT) | this paper | | Progenitors: DakAr-141069 NS2B3 sequence (GenBank EF105389) |
recombinant DNA reagent | Sylvatic (DakAr-141069) Dengue NS2B3 Protease (S135A) | this paper | | Progenitors: Sylvatic (DakAr-141069) Dengue NS2B3 Protease (WT) SDM product |
recombinant DNA reagent | human cDNA | this paper | | Progenitors: A549 cell line (ATCC CCL-185) |
recombinant DNA reagent | chimpanzee cDNA | this paper | | Progenitors: Coriell PR00748 |
recombinant DNA reagent | rhesus macaque cDNA | this paper | | Progenitors: Mm265-95 |
recombinant DNA reagent | marmoset cDNA | this paper | | Progenitors: Coriell PR07404 |
recombinant DNA reagent | mouse cDNA | this paper | | Progenitors: RNA extracted from mouse liver |
recombinant DNA reagent | human STING-HA (plasmid) | this paper | | Progenitors: human cDNA, pcDNA3.1 plasmid |
recombinant DNA reagent | human STING-HA (plasmid) | this paper | | Progenitors: human cDNA, pLPCX plasmid |
recombinant DNA reagent | human STING(R78W)-HA (plasmid) | this paper | | Progenitors: human STING-HA pcDNA3.1 SDM product |
recombinant DNA reagent | human STING(R78W)-HA (plasmid) | this paper | | Progenitors: human STING-HA pLPCX SDM product |
recombinant DNA reagent | human STING(R79D)-HA (plasmid) | this paper | | Progenitors: human STING-HA pcDNA3.1 SDM product |
recombinant DNA reagent | human STING(R78Q)-HA (plasmid) | this paper | | Progenitors: human STING-HA pcDNA3.1 SDM product |
recombinant DNA reagent | chimpanzee STING-HA (plasmid) | this paper | | Progenitors: chimpanzee cDNA, pcDNA3.1 plasmid |
recombinant DNA reagent | chimpanzee STING-HA (plasmid) | this paper | | Progenitors: chimpanzee cDNA, pLPCX plasmid |
recombinant DNA reagent | chimpanzee STING(W78R)-HA (plasmid) | this paper | | Progenitors: chimpanzee STING-HA pcDNA3.1 SDM product |
recombinant DNA reagent | chimpanzee STING(W78R)-HA (plasmid) | this paper | | Progenitors: chimpanzee STING-HA pLPCX SDM product |
recombinant DNA reagent | rhesus macaque STING-HA (plasmid) | this paper | | Progenitors: rhesus macaque cDNA, pcDNA3.1 plasmid |
recombinant DNA reagent | rhesus macaque STING(D79G)-HA (plasmid) | this paper | | Progenitors: rhesus macaque STING-HA pcDNA3.1 SDM product |
recombinant DNA reagent | marmoset STING-HA (plasmid) | this paper | | Progenitors: marmoset cDNA, pcDNA3.1 plasmid |
recombinant DNA reagent | marmoset STING(Q78R)-HA (plasmid) | this paper | | Progenitors: marmoset STING-HA pcDNA3.1 SDM product |
recombinant DNA reagent | mouse STING-HA (plasmid) | this paper | | Progenitors: mouse cDNA, pcDNA3.1 plasmid |
recombinant DNA reagent | mouse STING(Q78R)-HA (plasmid) | this paper | | Progenitors: mouse STING-HA pcDNA3.1 SDM product |
recombinant DNA reagent | mouse STING(93LRRG96)-HA (plasmid) | this paper | | Progenitors: mouse STING-HA pcDNA3.1 SDM product |
recombinant DNA reagent | human STING-3xFLAG (plasmid) | this paper | | Progenitors: human cDNA, pLPCX plasmid |
recombinant DNA reagent | human STING(R78W)-3xFLAG (plasmid) | this paper | | Progenitors: human STING-3xFLAG pLPCX SDM product |
recombinant DNA reagent | human STING(G230A)-3xFLAG (plasmid) | this paper | | Progenitors: human STING-3xFLAG pLPCX SDM product |
recombinant DNA reagent | human STING (R78W, G230A)-3xFLAG (plasmid) | this paper | | Progenitors: human STING-3xFLAG pLPCX SDM product |
recombinant DNA reagent | chimpanzee STING-3xFLAG (plasmid) | this paper | | Progenitors: chimpanzee cDNA, pLPCX plasmid |
recombinant DNA reagent | chimpanzee STING (W78R)-3xFLAG (plasmid) | this paper | | Progenitors: chimpanzee STING-3xFLAG pLPCX SDM product |
recombinant DNA reagent | chimpanzee STING(A230G)-3xFLAG (plasmid) | this paper | | Progenitors: chimpanzee STING-3xFLAG pLPCX SDM product |
recombinant DNA reagent | chimpanzee STING(W78R, A230G)-3xFLAG (plasmid) | this paper | | Progenitors: chimpanzee STING-3xFLAG pLPCX SDM product |
recombinant DNA reagent | IFN-ß1-luc (plasmid) | PMID: 21512573 | | |
recombinant DNA reagent | pRL-CMV (plasmid) | Promega: AF025843 | | Progenitors: pRL-null |
commercial assay or kit | Dual-Glo Luciferase Assay System | Promega | Cat#E2920 | |
commercial assay or kit | Superscript III First-Strand Synthesis System | Thermo Scientific | Cat#18080051 | |
software, algorithm | MEGA7 | | | http://www.megasoftware.net/ |
software, algorithm | ImageJ version 1.43u | | | http://rsb.info.nih.gov/ij |
software, algorithm | Python 2.7.11 | | | https://www.python.org |
software, algorithm | Sequencher | | | https://www.genecodes.com |