1. Ecology
  2. Evolutionary Biology
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CRISPR-based herd immunity can limit phage epidemics in bacterial populations

  1. Pavel Payne  Is a corresponding author
  2. Lukas Geyrhofer
  3. Nicholas H Barton
  4. Jonathan P Bollback  Is a corresponding author
  1. University of Liverpool, United Kingdom
  2. Institute of Science and Technology Austria, Austria
  3. Technion - Israel Institute of Technology, Israel
Research Article
Cite this article as: eLife 2018;7:e32035 doi: 10.7554/eLife.32035
11 figures, 4 tables, 1 data set and 2 additional files

Figures

Mechanism of CRISPR/Cas type II immunity.

The CRISPR/Cas system provides immunity to phages and its main features can be described by three distinct stages. (A) Acquisition. When a cell gets infected by a phage, a protospacer on the invading phage DNA (indicated as a red bar) is recognized by Cas1 and Cas2. The protospacer is cleaved out and ligated to the leader end (proximal to the Cas genes) of the CRISPR array as a newly acquired spacer (red diamond). (B) Processing. The CRISPR array is transcribed as a Pre-crRNA and processed by Cas9 (assisted by RNaseIII and trans–activating RNA, not shown) into mature crRNAs. (C) Interference. Mature crRNAs associate with Cas9 proteins to form interference complexes which are guided by sequence complementarity between the crRNAs and protospacers to cleave invading DNA of phages whose protospacers have been previously incorporated into the CRISPR array. (D) A truncated version of the CRISPR system on a low copy plasmid, which was used in this study lacks cas1 and cas2 genes and was engineered to target a protospacer on the T7 phage chromosome to provide Escherichia coli cells with immunity to the phage. The susceptible strain contains the same plasmid except the spacer does not target the T7 phage chromosome.

https://doi.org/10.7554/eLife.32035.003
Efficiency of bacterial resistance.

(A) The probability that a resistant cell bursts, relative to a susceptible cell, at three different initial multiplicities of infection (MOI). The probability that a resistant cell bursts at MOI 1000 is significantly higher than at MOI 10 (p = 0.019, t4 = 3.031) or at MOI 100 (p = 0.022, t5 = 2.674). The error bars show the standard deviations from the mean. Note that this measure is not a widely used ’efficiency of plating’ but it determines the probability of burst of single resistant cells (see Materials and methods for details). (B) The number of colony forming units (CFUs) post phage challenge (see Materials and methods). The mean number of CFUs after the bacterial cultures were exposed to the phage is not significantly different between susceptible and resistant strains at MOI 10 (p = 0.239, t22 = 0.721) and (C) at MOI 100 (p = 0.27, t30 = 1.124), indicating that the resistant cells’ growth is halted after the cells are infected by a phage. The error bars show the standard deviations from the mean. There were no detectable CFUs in either susceptible or resistant cell cultures at MOI 1000. It should be noted that the indicated MOI values do not correspond to the average number of phages that adsorb to cells in the experiments. For MOI 10 we estimated the mean number of phages per cell as 0.229 and for MOI 100 as 0.988 (see Materials and methods for details). It was impossible to determine the mean for MOI 1000 as there were no detectable CFUs under such conditions. The data presented in this figure can be found in Figure 2—source data 1.

https://doi.org/10.7554/eLife.32035.004
Figure 2—source data 1

Efficiency of bacterial resistance.

https://doi.org/10.7554/eLife.32035.005
Fraction of surviving populations at 18h post phage infection.

Bacterial populations consisting of various fractions of resistant to susceptible individuals infected with 50 phages, corresponding to a multiplicity of infection (MOI) of 10-4, designed to resemble an epidemic initiated by the burst size from one infected individual (see Table 2 for burst size estimates). Each population phage challenge is replicated 16 times. The solid dark green line shows the model prediction, Equation 4, for the herd immunity threshold (H), given latent period (λ), bacterial growth rate (α), and phage burst size (β). Shaded area indicates ±1 standard deviation. The data presented in this figure can be found in Figure 3—source data 1.

https://doi.org/10.7554/eLife.32035.006
Figure 3—source data 1

Fraction of surviving populations at 18 hr post phage infection.

https://doi.org/10.7554/eLife.32035.007
Measuring bacterial growth without phage.

(A) Trajectory of population size on agar plates over time. For modeling, we assume two states of growth (dashed brown curve): first, the bacterial population grows exponential until the time Tdepl, when nutrients are depleted. From this time on, growth rate is assumed to be zero and the population saturates at a maximal size Bfinal. Experimental observations fit this proposed growth curve to a very good extent. After all, half of all nutrients are used up in the last generation indicating that the switch between growth and no-growth should be fast. (B) Growth rates of bacteria in diluted medium follow closely Monod’s empirical law, given by expression Equation 9. Fit parameters are found to be αmax0.720h-1 and Kc0.257 (with the latter in dimensionless units as dilution of LB medium), see also Table 1. The data presented in this figure can be found in Figure 4—source data 1.

https://doi.org/10.7554/eLife.32035.008
Figure 4—source data 1

Bacterial growth on soft agar plates (tab Figure 4A) and bacterial growth in LB medium of various dilutions (tab Figure 4B).

https://doi.org/10.7554/eLife.32035.009
Herd immunity threshold in liquid culture as a function of bacterial growth.

(A) Phage burst size (β) change as a function of nutrient concentrations. (B) Latent period (λ) increase across the range of nutrient concentrations. Values for β and λ are given in Table 2. (C) Population survival analysis upon phage challenge as a function of the fraction of resistant cells and the intrinsic growth rate (nutrient availability, N). Bacteria survive the phage infection (full circles), collapse (empty circles), or exhibit both outcomes (circled dots) in the 16 to 18 replicates, done in 3 independent batches. Light green errorbars at investigated dilutions of LB show the expected value and its standard deviation of H(α), Equation 5, with standard error propagation of the measured β, λ and α. In order to interpolate herd immunity to dilutions N not probed in experiments (dark green line), we use a second order polynomial in N to fit the data for both β/λ and β, which excellently matches the average measurements (a naive linear fit displays non-negligible deviations and non-sensical negative values). In addition, the dependence α=α(N) is obtained by numerically inverting the Monod growth rate dependence, see Equation 9. The data presented in this figure can be found in Figure 5—source data 1 and Figure 5—source data 2.

https://doi.org/10.7554/eLife.32035.010
Figure 5—source data 1

Figure 5A B source data: Phage burst sizes and latent period in different dilutions of the growth medium.

https://doi.org/10.7554/eLife.32035.011
Figure 5—source data 2

Figure 5C: Fraction of surviving populations in different dilutions of the growth medium.

https://doi.org/10.7554/eLife.32035.012
Properties of expanding phage epidemics on bacterial lawns.

(A) Example of plaque morphology and size change over 48 hours for populations with 50% resistant cells (top) and a control with 100% susceptible cells (bottom). (B) Mean plaque size area through time. Colors indicate the different fraction of resistant individuals (color coding as in panel C). Note the distinct two phases of plaque growth – initially, phage grow fast with exponentially growing bacteria but slow once the nutrients are depleted (10 hr). The plaque radius is reduced, relative to 100% susceptible population, even when only a small fraction of resistant individuals are in the population. (C) Final plaque radius at 48 hpi. Green line shows the prediction from the model for the plaque radius r. Grey numbers indicate the number of plaques measured. Error bars indicate the standard deviations. The data presented in this figure can be found in Figure 6—source data 1.

https://doi.org/10.7554/eLife.32035.014
Figure 6—source data 1

Plaque radii for all population compositions and time points.

https://doi.org/10.7554/eLife.32035.015
Speed of phage epidemic expansion on bacterial lawns.

(A) Speed of expanding phage epidemics for all population compositions is initially high, before it drops once nutrients are depleted at around 10hpi (hours post infection). (B) Plaque speed significance. Comparing speeds of plaque spread with the 100% susceptible control. Linear regression of a sliding window spanning 4 hours of the radius sizes was calculated for all individual plaques and all compositions of the populations between t0 and t24. Slopes of the linear regressions for all compositions of the populations were compared using a two-sided heteroscedastic t-test against the 100% susceptible dataset. The data presented in this figure can be found in Figure 7—source data 1.

https://doi.org/10.7554/eLife.32035.016
Figure 7—source data 1

Speed of plaque expansion in populations consisting of varying proportions of resistant to susceptible bacteria.

https://doi.org/10.7554/eLife.32035.017
Estimating diffusion constant of phages.

(A), (B) Phage are slowly expanding on agar which can be observed via their fluorescence. Pictures are taken 5h apart. (C) The diffusion constant D can be estimated as best-fit parameter in a heat kernelK(D) propagates the fluorescence profile L(t) at time t forward (via a convolution to “smear” out the signal) to the profile L(t+Δt) at the next measured time point. The difference between the expected change and the actual profile is quantified as total squared deviation, see Equation 10, which we minimize to obtain D. Consequently, we can estimate the diffusion constant as D1.1710-2mm2/h. The green line uses this estimated parameter D and shows the change between the profile at t=10h (orange line) and the profile at t=15h (light brown line), assuming diffusive spread of phages. See Materials and methods for more information.

https://doi.org/10.7554/eLife.32035.019
Appendix 1—figure 1
Simulated trajectories for all populations in liquid culture for the extended model, including infected and recovering bacteria.

Trajectories are obtained by numerically integrating equations Equation 12, using parameters listed in Appendix 1—table 1 and additionally N=1, Y=210-10cells-1, δ=10-7h-1 and ρ=1.5h-1. (A) For population compositions with a large majority of resistant cells (S=10-3), phages get wiped out fast. (B) For intermediate S (close to parameters where we observe both, collapsed and surviving, populations, see Figure 3), the populations exhibit a complex, non-monotonic trajectory. After fast initial growth of phages, bacterial populations decay but ultimately can recover. (C) If the fraction of susceptibles is too large (S=0.06), the whole bacterial population is infected and succumbs to the overwhelming phage infection. See supporting text for more detailed information.

https://doi.org/10.7554/eLife.32035.023
Appendix 2—figure 1
Number of plaques declines faster than proportionally to the fraction of resistant bacteria.

Number of plaque forming units observed on a plate (y-axis) for different proportions of resistant bacteria (x-axis). Grey numbers below each boxplot indicate the average number of phages inoculated in the respective treatment. The numbers of phages inoculated were chosen to retain the expected number of plaques on the plate (green dashed line) as in the 0% resistant treatment (red boxplot). Plates were prepared using identical procedure as in Time-lapse imaging of plaque growth (see Materials and methods). The data presented in this figure can be found in Appendix 2—figure 1—source data 1.

https://doi.org/10.7554/eLife.32035.026
Appendix 2—figure 1—source data 1

Measurements of plaque numbers in populations consisting of varying proportions of resistant to susceptible bacteria.

https://doi.org/10.7554/eLife.32035.027
Appendix 3—figure 1
Image of the scanner system.

Photograph of the scanner system used for time-lapse imaging of phage spread in spatially structured bacterial populations. Three scanners (Epson Perfection V600 Photo Scanner) simultaneously scanned 12 plates in total every 20 min in 30C for 48 hr per experiment.

https://doi.org/10.7554/eLife.32035.029

Tables

Table 1
Estimated parameters for bacterial growth using Monod kinetics.

Undiluted LB medium (N=1) is assumed to have 15mg/ml nutrients (10mg/ml Tryptone, 5mg/ml yeast extract). The full dataset is shown in Figure 4.

https://doi.org/10.7554/eLife.32035.013
EstimateUnits
αmax0.720(± 0.011)[h-1]
Kc0.257(± 0.012)Dilution N of LB [01]
Table 2
Estimated parameters for phage growth.

See also Figure 5A,B.

https://doi.org/10.7554/eLife.32035.018
MediumDilutionLatent periodBurst sizeBurst size/hour
Nλ[min]ββ/λ[h-1]
LB 00.0101.1 (±10.9)3.0 (±1.9)1.8 (±1.1)
LB 200.243.4 (±3.9)3.0 (±1.9)16.6 (±6.0)
LB 500.540.0 (±3.0)35.6 (±16.4)53.4 (±24.9)
LB 1001.036.1 (±6.1)85.6 (±47.3)142.1 (±82.1)
Key resources table
Table of key strains, reagents and software used in this study.
https://doi.org/10.7554/eLife.32035.020
Reagent type (species)
or resource
DesignationSource or referenceIdentifiersAdditional information
gene (Streptococcus
pyogenes SF370)
cas9National Center for
Biotechnology Information
NCBI:NC_002737.2;
gene_ID:901176;
RRID:SCR_006472
Gene symbol SPy_1046
strain, strain background
(Escherichia coli)
E. coli K12 MG1655Own collectionNA
strain, strain background
(Bacteriophage T7)
E. coli bacteriophage T7ATCC CollectionATCC:BAA-1025-B2;
RRID:SCR_001672
recombinant DNA reagentpCas9Addgene Vector DatabaseAddgene:42876;
RRID:SCR_005907
pCas9 plasmid was a gift
from Luciano Marraffini
recombinant DNA reagentpCas9T7resistantthis paperNAPlasmid derived from pCas9
commercial assay or kitPureYield Plasmid
Miniprep System
PromegaPromega:A1223;
RRID:SCR_006724
chemical compound, drugChloramphenicolSigma-AldrichSigma-Aldrich:C0378-5G;
RRID:SCR_008988
software, algorithmPerkinElmer Volocity v6.3RRID:SCR_002668Volocity 3D Image
Analysis Software
software, algorithmFiji v1.0doi: 10.1038/nmeth.2019RRID:SCR_002285Image processing
package of ImageJ
software, algorithmRStudio 1.0.153RRID:SCR_000432Software for the R
statistical computing
software, algorithmPython 3.6.3RRID:SCR_008394Python programming language
software, algorithmModel source codedoi: 10.5281/zenodo.1038582RRID:SCR_004129Zenodo repository
Appendix 1—table 1
Parameters used in simulations shown in Appendix 1—figure 1.
https://doi.org/10.7554/eLife.32035.024
ParameterValueUnitsComment
Bacterial growth ratea0.631/hTable 1, Figure 4
YieldY210-11/cellMeasured in dilution of LB
Recovery rateρ1.51/hSee this appendix
Adsorption constantδ10-71/(hcell)See this appendix
Diffusion constantD1.1710-2mm2/hSee Methods
Burst sizeβ85.6phages/cellTable 2, Figure 5
Latency timeλ0.60hTable 2, Figure 5
Initial bacterial populationB0105cells
Initial phage populationP010phages

Data availability

The following data sets were generated
  1. 1
    Data from: CRISPR-based herd immunity limits phage epidemics in bacterial populations
    1. Payne P
    2. Geyrhofer L
    3. Barton NH
    4. Bollback JP
    (2017)
    Available at Dryad Digital Repository under a CC0 Public Domain Dedication.

Additional files

Transparent reporting form
https://doi.org/10.7554/eLife.32035.021
Appendix 2—figure 1—source data 1

Measurements of plaque numbers in populations consisting of varying proportions of resistant to susceptible bacteria.

https://doi.org/10.7554/eLife.32035.027

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