Cell-type heterogeneity in the early zebrafish olfactory epithelium is generated from progenitors within preplacodal ectoderm
- Université de Toulouse, France
- University of California, United States
Figures
Figure 1 with 1 supplement
Islet1/2 labels Gnrh3 neurons of the olfactory epithelium and its expression is unaffected in sox10 mutants.
(A) Single confocal sections of olfactory epithelia from Tg(gnrh3:eGFP) embryos at 48 hpf, in either wildtype or sox10t3/t3 mutants, labelled for the expression of Islet1/2 (magenta) and eGFP …
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Figure 1—source data 1
Islet+ cell number quantification.
- https://doi.org/10.7554/eLife.32041.004
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Figure 1—source data 2
eGFP+ cell number quantification.
- https://doi.org/10.7554/eLife.32041.005
Figure 1—figure supplement 1
Expression of endogenous gnrh3 in sox10t3/t3 mutants and wildtype siblings.
Representative bright-field images of in situ hybridisation against endogenous gnrh3 transcripts in wildtype or sox10t3/t3 mutant olfactory epithelia showing high and low numbers of expressing …
Figure 2 with 1 supplement
Cre/lox lineage mapping of neural crest cells reveals contributions to cells surrounding the olfactory epithelium but not to Islet/Gnrh3+ neurons.
Single confocal sections of an olfactory epithelium from a double-heterozygous Tg(−28.5Sox10:Cre);Tg(ef1a:loxP-DsRed-loxP-egfp) embryo at 48 hpf, labelled for the expression of Islet1/2 (magenta) …
Figure 2—figure supplement 1
Expression of eGFP is detected throughout the head after switching of the loxP-DsRed-loxP-egfp cassette, including in known neural crest-derived lineages.
(A) Low magnification images of the entire head of a double-heterozygous Tg(−28.5Sox10:Cre);Tg(ef1a:loxP-DsRed-loxP-egfp) embryo at 48 hpf labelled for eGFP (green); nuclei are labelled with DAPI …
Figure 3 with 1 supplement
Lineage reconstruction reveals an anterior preplacodal ectoderm origin for Gnrh3 neurons: backtracking.
(A) Schematic representation of the backtracking strategy. Synthetic mRNAs encoding Histone2B-RFP (H2B-RFP) were injected into Tg(gnrh3:eGFP) transgenic embryos, which were subsequently imaged from …
Figure 3—figure supplement 1
Backtracking data from individual Tg(gnrh3:eGFP) embryos.
Confocal projections extracted from 4D datasets at 36 and 12 hpf for 4 embryos analysed and not shown in Figure 3 showing the GFP channel alone, and the position of the backtracked nuclei of …
Figure 4
Lineage reconstruction reveals an anterior preplacodal ectoderm origin for Gnrh3 neurons: photoconversion.
(A,B) Confocal projections of NLS-mEos2 loaded, Tg(gnrh3:eGFP) embryos at 12 hpf before (A) and after photoconversion (B). The first panel in (A) shows a schematic representation of the anterior …
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Figure 4—source data 1
gnrh3:eGFP+ and mEos2PC+ cell number quantification.
- https://doi.org/10.7554/eLife.32041.013
Figure 5 with 2 supplements
Microvillous sensory neurons derive from preplacodal precursors: backtracking.
(A–C) Confocal projections from a representative 4D dataset at 36, 28, 20 and 12 hpf showing the position of the nuclei backtracked from 10 sox10:eGFP-negative (A, pink), 10 lens (B, yellow) and 10 …
Figure 5—figure supplement 1
Expression of endogenous trpc2b is unchanged in sox10t3/t3 mutant embryos relative to wildtype siblings.
Representative bright-field images of in situ hybridisation against endogenous trpc2b transcripts in wildtype or sox10t3/t3 mutant epithelia showing high and low numbers of expressing cells or no …
Figure 5—figure supplement 2
Backtracking data from individual Tg(sox10:eGFP) embryos.
Confocal projections extracted from 4D datasets at 36 and 12 hpf for 3 embryos analysed and not shown in Figure 5 showing the position of the backtracked nuclei of sox10:eGFP-negative (pink) and …
Figure 6
Microvillous sensory neurons derive from preplacodal precursors: photoconversion.
(A,B) Confocal projections of NLS-mEos2 loaded, Tg(−4.9sox10:eGFP) embryos at 12 hpf before (A) and after photoconversion (B). The first panel in (A) shows a schematic representation of the anterior …
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Figure 6—source data 1
sox10:eGFP+ and mEos2PC+ cell number quantification.
- https://doi.org/10.7554/eLife.32041.020
Videos
Video 1
Lineage reconstruction reveals an anterior preplacodal ectoderm origin for Gnrh3 neurons: backtracking.
Movie showing an acquisition series and backtracking of a Gnrh3 neuron in a Histone2B-RFP loaded Tg(gnrh3:eGFP) transgenic embryo. The movie is divided into 5 parts: an acquisition phase, a phase …
Video 2
Lineage reconstruction reveals an anterior preplacodal ectoderm origin for Gnrh3 neurons: tracks.
Movie showing an acquisition series and backtracking of gnrh3:eGFP-negative olfactory cells, lens cells and gnrh3:eGFP-positive olfactory cells in an Histone2B-RFP loaded Tg(gnrh3:eGFP) transgenic …
Video 3
Microvillous sensory neurons derive from classical preplacodal precursors: backtracking.
Movie showing an acquisition series and backtracking of a microvillous sensory neuron in a Histone2B-RFP loaded Tg(sox10:eGFP) transgenic embryo. The movie is divided into 5 parts: an acquisition …
Video 4
Microvillous sensory neurons derive from classical preplacodal precursors: tracks.
Movie showing an acquisition series and backtracking of sox10:eGFP-negative olfactory cells, lens cells and sox10:eGFP-positive olfactory cells in an Histone2B-RFP loaded Tg(sox10:eGFP) transgenic …
Additional files
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Transparent reporting form
- https://doi.org/10.7554/eLife.32041.021
