The human CaP cells DU145, PC3, and LNCaP were seeded in 10% FBS and harvested after two days to measure various parameters. (A) Immunoblot showing the expression levels of PTEN, P-Akt (ser 473), …
The data represent the mean ±SEM of three independent quantification experiments performed in triplicate. *p<0.05 versus DU145.
The data represent the mean ±SEM of three independent quantification experiments performed in triplicate. ***p<0.0001 versus DU145. (1s4-5) DU145 cells were transiently transfected with hSesn3 or …
The data represent the mean ±SEM of three independent experiments performed in triplicate. *p=0.02, **p=0.01 versus the control for each cell line.
(A) CaP cell lines were incubated with 2-ME for 24 hr, the cells were fixed and apoptosis was quantified by DAPI staining. The data represent the mean ±SEM of three independent experiments performed …
The data represent the mean ±SEM of two independent experiments performed in duplicate.
The data represent the mean ±SEM of two independent experiments performed in quadruplicate. (Right) Cell death was assessed on fixed cells by DAPI staining after drug treatment: 2-ME 1 µM (20 hr) or …
The data represent the mean ±SEM of three independent experiments performed in triplicate.
The data represent the mean ±SEM of three measurements performed in duplicate.
The data represent the mean ±SEM of three independent experiments performed in triplicate. *p<0.05, **p<0.01 versus the control for each cell line.
The graphs represent the cell death measured by PI staining (Left) or ROS levels after incubation with H2DCFDA (Right). The data represent the mean ±SEM of three independent experiments performed in …
PTEN was downregulated in DU145 cells (1: control shLacZ, 2: shPTEN) or overexpressed in PC3 and LNCaP cells (3: control pBP, 4: pBP-PTEN). (A, B) Relative ROS levels: cells were incubated with …
The data represent the mean ±SEM of three independent experiments performed in triplicate. *p<0.05, **p<0.001 versus the control for each cell line. ##p<0.05 versus PEITC.
Cells were then incubated for 17 hr with PEITC or rapamycin/PEITC before measurement of relative cytosolic ROS level (Left) or cell death (Right).
Akt1 and Akt2 were knocked down in PC3 and LNCaP cells. Once cell lines were established, mAkt was re-expressed in these cells. Cells were incubated with PEITC for 17 hr, and then cytosolic ROS …
(A–C) DU145 (A), PC3 (B), and LNCaP cells (C) were treated with rapamycin (100 nM). Total cell extracts were prepared at different time points as indicated and subjected to immunoblotting with …
When required, CaP cells were incubated with 20 nM rapamycin (RAPA) for 8 hr before the addition of PEITC (3 µM). After 17 hr of incubation with PEITC (±RAPA), the ROS levels in live cells after …
PC3 and LNCaP cells were incubated for 8 hr with rapamycin (RAPA, 20 nM) or torin (250 nM) before measurement of the OCR (Left) or cytoplasmic ROS levels (Right). The data represent the mean ±SEM of …
Twenty-four nude mice were injected subcutaneously with DU145 cells in both flanks and randomly divided into four groups (four mice per group, eight tumors per group) for treatment with PEITC, …
(A) Tissue lysates were prepared from prostates isolated from four control mice (Ptenf/f or Pbsn-Cre4) and four Pbsn-Cre4;Ptenf/f mice. Immunoblot analysis shows the expression levels of PTEN, Akt-P …
The numbers of treated mice in the control group were vehicle (n = 6), rapamycin (RAPA, n = 12), PEITC (n = 8), and RAPA +PEITC (n = 8), and the numbers of treated mice in the Pbsn-Cre4;Ptenf/f …
The box plots represent the 25th to 75th percentiles (boxes) with the median, and the whiskers represent the maximum and minimum values. No significant differences were detected.
Representative images of different prostate tumor grades in the anterior lobe of the prostate of untreated mice (-), rapamycin +PEITC-, and NAC-treated mice. The individual images were derived from …
(A) Schematic of mice treatment: control and Pbsn-Cre4;Ptenf/f mice were randomly divided into four groups of four to 10 mice at 2 months of age, and they received IP drug injections as indicated in …
The numbers of treated mice in the control group were vehicle (n = 5), RAPA (n = 5), PEITC (n = 5), and RAPA +PEITC (n = 7), and the numbers of treated mice in the Pbsn-Cre4;Ptenf/f group were …
(A) DU145, PC3, and LNCaP cells were treated with MK-2206 (0.5 μM - 24 hr) to inhibit Akt. The immunoblot shows the protein levels of P-Akt, total Akt, HK2, and ß-actin as a loading control. (B–G) …
The immunoblot shows the expression levels of HK1, HK2, and actin as a loading control in PC3 control, HK1 knockdown, HK2 knockdown, and double HK1 and HK2 knockdown cells. The graph shows the total …
The data represent the mean ±SEM of three independent experiments performed in triplicate. ***p<0.0001 versus LacZsh cells on day 6.
(A) After mAkt overexpression, DU145 cells were treated with etoposide for 24 hr before cell death was assessed by PI staining on live cells with Celigo Image cytometer. (B) Akt1 and Akt2 were …
(A) Graphs showing the relative xenograft tumor weights of mice treated with control diet/vehicle, control diet/etoposide, DOX diet/vehicle, and DOX diet/etoposide. The data represent the average …
PC3 cells expressing an inducible control (Scr) or HK2 shRNA were exposed to 900 ng/ml DOX for 5 days for HK2 deletion before analysis. ECAR was measured after HK2 deletion using the Seahorse XF96e …
PC3 cells expressing an inducible control (Scr) or HK2 shRNA were exposed to 900 ng/ml DOX for 5 days for HK2 deletion before analysis. OCR was measured after HK2 deletion using the Seahorse XF96e …
PC3 cells expressing an inducible control (Scr) or HK2 shRNA were exposed to 900 ng/ml DOX for 5 days for HK2 deletion before analysis. Cells were incubated with H2DCFDA, and the level of …
PC3 cells expressing an inducible control (Scr) or HK2 shRNA were exposed to 900 ng/ml DOX for 5 days for HK2 deletion before analysis. After HK2 knockdown with DOX, cells were treated with PEITC …
(A) Tissue lysates were prepared from prostates isolated from three control mice (Ptenf/f;HK2f/f), three Pbsn-Cre4;Ptenf/f mice and three Pbsn-Cre4;Ptenf/f;HK2f/f/ mice. The immunoblot shows the …
At end-point, PI and Hoechst 33342 were added to wells for 30min and plates were visualized with Celigo Image Cytometer, and cell death was calculated.
Grade | |||||
---|---|---|---|---|---|
No PIN | Low grade PIN | High grade PIN | Microinvasive carcinoma | Invasive carcinoma | |
Pbsn-Cre4;Ptenf/f* | 66% | 33% | |||
Pbsn-Cre4;Ptenf/f R + P† | 33% | 16% | 33% | 16% | |
Pbsn-Cre4;Ptenf/f + NAC‡ | 25% | 75% |
*The anterior lobes of prostates from untreated mice were analyzed by histopathology at 8 months (percentage of mice with highest grade is indicated).
†The anterior lobes of prostates from mice treated at 4 months with rapamycin and PEITC (R + P) were analyzed by histopathology at 8 months (percentage of mice with highest grade is indicated).
‡The anterior lobes of prostates from mice treated at 4 months with NAC were analyzed by histopathology at 8 months (percentage of mice with highest grade is indicated).