Neuronal activity determines distinct gliotransmitter release from a single astrocyte
- University of Minnesota, United States
Figures
Figure 1
Unitary CA3-CA1 synapse recordings from hippocampal pyramidal neurons.
(A) Fluorescent images showing a pyramidal cell expressing GFP (green) and an astrocyte loaded with Texas Red (red) through the recording pipette. Scale bars: 20 µm. (B) Schematic drawing depicting …
Figure 2 with 2 supplements
A single synapse can be modulated by different gliotransmitters.
(A) Schematic drawing depicting paired recordings from CA1 pyramidal neurons and the stimulating electrodes (S1 and S2). (B) Fluorescent image showing two pyramidal cells, one loaded with Texas Red …
Figure 2—figure supplement 1
eCBs mediate glutamate-induced synaptic potentiation but not heterosynaptic depression.
(A) Schematic drawing depicting paired recordings from two CA1 pyramidal neurons and the stimulating electrode. (B) Representative EPSC traces before and after neuronal depolarization (ND) recorded …
Figure 2—figure supplement 2
Astroglial specific GFAP-CB1-null mice shows impairments in astrocytic responsiveness to CB1 receptor agonists but not in the neuronal CB1 receptor dependent depolarization-induced suppression of inhibition (DSI).
(A) Scheme depicting local application of WIN55,212–2 (500 µM) and ATP (2 mM) through glass pipettes. (B) Pseudocolor images showing the fluorescence intensities of Fluo4-AM of 2 astrocytes recorded …
Figure 3 with 3 supplements
Astrocytes differentially modulate synaptic transmission in response to neuronal activity.
(A) Schematic drawing depicting paired recordings from a CA1 pyramidal neuron and a S. R. interneuron and the stimulating electrode. Representative traces showing the interneuron depolarization …
Figure 3—figure supplement 1
Baclofen application mimics interneuron depolarization.
(A) Scheme depicting the recorded CA1 pyramidal neuron, the SC stimulating electrode and the local application of baclofen (200 µM). (B) Calcium event probability in response to baclofen (n = 46 …
Figure 3—figure supplement 2
Astroglial specific GLAST-GABAB-null shows impairments in astrocytic responsiveness to GABAB receptor agonists but not in the neuronal responsiveness.
(A) Scheme depicting local application of baclofen (200 µM) and ATP (2 mM) through glass pipettes. (B) Pseudocolor images showing the fluorescence intensities of Fluo4-AM of 2 astrocytes recorded …
Figure 3—figure supplement 3
The biphasic synaptic regulation is not cell type specific.
(A) Schematic drawing depicting paired recordings from a CA1 pyramidal neuron and a PV+ interneuron (red) and the stimulating electrode. (B) Fluorescence image showing PV+ interneurons in the CA1 …
Figure 4 with 1 supplement
Astrocytes release glutamate and ATP/adenosine in response to neuronal activity.
(A) Synaptic parameters vs time in the presence of CPT (2 µM) or LY367385 (100 µM). Zero time correspond with the beginning of the IN depolarization train (arrow). The open circles show the synaptic …
Figure 4—figure supplement 1
Astrocytes co-release glutamate and ATP.
(A) Number of cells that underwent synaptic potentiation, depression, both or no change after IN depolarization. (B) Synaptic parameters vs time in control conditions (n = 5) and in the presence of …
Figure 5
A single astrocyte regulates single synapses downstream of the GABAergic activity.
(A) Schematic drawing depicting paired recordings from one pyramidal neuron and one astrocyte filled with NP-EGTA, GDPβS filling the astrocytic network, and the stimulating electrode. (B) …
Figure 6
Frequency and stimulus duration dependence of gliotransmitter release.
(A) Schematic illustration of the astrocyte-induced synaptic modulation and the parameters measured. (B) Percentage of effect (potentiation or depression) when the interneurons were depolarized at …
Figure 7
Schematic of the signaling pathways involved in gliotransmitter release.
(A) Low frequency or short interneuron stimulation induces glutamate release from astrocytes leading to a potentiation of neurotransmitter release. (B) High frequency or prolonged interneuron …
Additional files
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Transparent reporting form
- https://doi.org/10.7554/eLife.32237.015
