Codon usage bias controls mRNA and protein abundance in trypanosomatids
Abstract
Protein abundance differs from a few to millions of copies per cell. Trypanosoma brucei presents an excellent model for studies on codon bias and differential gene expression because transcription is broadly unregulated and uniform across the genome. T. brucei is also a major human and animal protozoal pathogen. Here, an experimental assessment, using synthetic reporter genes, revealed that GC3 codons have a major positive impact on both mRNA and protein abundance. Our estimates of relative expression, based on coding sequences alone (codon usage and sequence length), are within 2-fold of the observed values for the majority of measured cellular mRNAs (n>7000) and proteins (n>2000). Our estimates also correspond with expression measures from published transcriptome and proteome datasets from other trypanosomatids. We conclude that codon usage is a key factor affecting global relative mRNA and protein expression in trypanosomatids and that relative abundance can be effectively estimated using only protein coding sequences.
Data availability
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SILAC proteome data#6nVGofIEQu6D4odoX8aAd- odoX8aAdpUngsx1fAv43g8UN2w23Bb/ dXd6zBwaqq7SQKQcH7Mf05dGtfye0vl8pnrH3mu- ce8eA67EAAAAAAAAcbw = = ).
Article and author information
Author details
Funding
Wellcome (100320/Z/12/Z)
- David Horn
Wellcome (203134/Z/16/Z)
- David Horn
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Dominique Soldati-Favre, University of Geneva, Switzerland
Version history
- Received: October 3, 2017
- Accepted: March 9, 2018
- Accepted Manuscript published: March 15, 2018 (version 1)
- Version of Record published: April 11, 2018 (version 2)
Copyright
© 2018, Jeacock et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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