Figures and data in Hypocretin underlies the evolution of sleep loss in the Mexican cavefish

7 figures and 1 additional file

Figures

Figure 1 with 3 supplements

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Figure 1—source data 1 Hypocretin transcript and peptide levels are elevated in Pachón cavefish. This source file includes the analysis for comparisons between surface and Pachón cavefish for sleep duration (Figure 1B), hcrt expression (Figure 1C), the number of cells expression HCRT (Figure 1D), and the intensity of HCRT signal (Figure 1E).: https://doi.org/10.7554/eLife.32637.006
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Figure 1—figure supplement 1

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Figure 1—figure supplement 1—source data 1 Quantification of qPCR housekeeping genes and fluorescent intensity between surface and cavefish. This source file includes quantification of qPCR differences for the housekeeping gene Gadph (Figure 1—figure supplement 1B) and comparison of fluorescence intensity between surface and Pachón fry (Figure 1—figure supplement 3C).: https://doi.org/10.7554/eLife.32637.007
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Figure 1—figure supplement 2

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Figure 1—figure supplement 3

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Figure 2

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Figure 2—source data 1 Pharmacological inhibition of HCRT Receptor two promotes sleep in Pachón cavefish. Data analysis comparing the effects of 1 um and 10 um TCS compared to controls in surface fish and cavefish for sleep (Figure 2C), waking activity (Figure 2D), total bout number (Figure 2E), and average bout duration (Figure 2F).: https://doi.org/10.7554/eLife.32637.009
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Figure 3 with 2 supplements

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Figure 3—source data 1 Panel of pharmacological HCRT antagonists reveals wake-promoting role of HCRT in juvenile cavefish. This source file includes analysis of treatment with the HCRTR inhibitors Suvorexant, TCS, and EMPA on total sleep duration (Figure 3B), waking activity (Figure 3C), total number of sleep bouts (Figure 3D), and average sleep bout duration (Figure 3E).: https://doi.org/10.7554/eLife.32637.013
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Figure 3—figure supplement 1

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Figure 3—figure supplement 1—source data 1 Inhibition of HCRT signaling increases sleep in larval cavefish. This source file includes analysis of 1 um and 10 um TCS treatment on total sleep duration in surface fish and Pachón cavefish (Figure 3—figure supplement 1B).: https://doi.org/10.7554/eLife.32637.014
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Figure 3—figure supplement 2

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Figure 3—figure supplement 2—source data 2 HCRT agonist partially alters sleep behavior in juvenile surface fish, but not Pachón cavefish. This source data file includes quantification of the effects of the HCRT agonist YNT-185 on total sleep duration (Figure 3—figure supplement 2A), waking activity (Figure 3—figure supplement 2B), total bout number (Figure 3—figure supplement 2C), and average bout duration (Figure 3—figure supplement 2D).: https://doi.org/10.7554/eLife.32637.015
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Figure 4 with 1 supplement

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Figure 4—source data 1 Transient knockdown of hcrt increases sleep in 4 days post fertilization cavefish. This source file includes analysis comparing the effects of hcrt morpholino treatment on sleep duration (Figure 3A) waking activity (Figure 3B), total number of sleep bouts (Figure 3C), and average sleep bout duration (Figure 3D).: https://doi.org/10.7554/eLife.32637.017
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Figure 4—figure supplement 1

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Figure 4—figure supplement 1—source data 1 The effect of morpholino injection on survival in surface fish and cavefish. This source file includes data analysis quantifying the effects of morpholino injection on survival in surface fish (Figure 4—figure supplement 1A), and Pachón cavefish; Figure 4—figure supplement 1B over the first 96 hr post fertilization.: https://doi.org/10.7554/eLife.32637.019
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Figure 5 with 1 supplement

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Figure 5—source data 1 Genetic silencing of subsets of HCRT neurons selectively increases sleep in Pachón cavefish. This file contains analyses quantifying the effects of HCRT neuron silencing on total sleep duration (Figure 5E), and waking activity (Figure 5F). The regression analysis determining the relationship between the number of HCRT neurons silenced and total sleep duration is depicted in Figure 5G.: https://doi.org/10.7554/eLife.32637.022
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Figure 5—figure supplement 1

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Figure 5—figure supplement 1—source data 1 Genetic silencing of subsets of HCRT alters sleep architecture in Pachón cavefish. This source file contains analysis of total bout number (Figure 5—figure supplement 1A), and bout duration (Figure 5—figure supplement 1B) in fish with genetically silenced HCRT neurons.: https://doi.org/10.7554/eLife.32637.023
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Figure 6

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Figure 6—source data 1 Chemical ablation of mechanosensory lateral line reduces HCRT levels in Pachón cavefish. This source file contains analysis of the effects of lateral line ablation with gentamicin on hcrt expression (Figure 6E), HCRT cell number (Figure 6F), and HCRT fluorescence levels (Figure 6G).: https://doi.org/10.7554/eLife.32637.025
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Figure 7

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Figure 7—source data 1 Starvation selectively inhibits HCRT levels in cavefish. This source file contains analysis quantifying the effects of starvation on hcrt expression (Figure 7A), HCRT cell number (Figure 7B), and HCRT fluorescence levels (Figure 7C).: https://doi.org/10.7554/eLife.32637.027
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