Jak-Stat pathway induces Drosophila follicle elongation by a gradient of apical contractility
- Université Clermont Auvergne - CNRS UMR 6293- INSERM U1103, France
Figures
Figure 1 with 1 supplement
Polar cells determine the axis of early elongation.
(a) WT ovariole illustrating follicle elongation during the early stages of oogenesis (stages 2–6). (b) Optical cross-section of a stage 7 WT follicle stained with FasIII, a polar cell marker …
Figure 1—figure supplement 1
Polar cells determine the axis of early elongation.
(a) Elongation coefficients corresponding to the slope of the regression lines of the plots in (b). (b) Plot of the long axis as a function of a short axis of (b) fat2 mutant follicles (red = WT, …
Figure 2 with 1 supplement
Upd is a polarizing cue for early elongation.
(a) Optical cross-section of a WT stage 7 follicle stained with FasIII (polar cell marker, white) and F-actin (red). (b) Expression of the 10xStatGFP reporter showing the progressive formation of a …
Figure 2—figure supplement 1
JAK-STAT singalling and early elongation.
(a–c) Representative follicles expressing 10XStatGFP in (a) WT (b) upd RNAi or (c) Stat92E RNAi background. (d) Quantification of the Stat activity gradient for the indicated genotypes at stage 7 …
Figure 3 with 1 supplement
Myosin II is required for early elongation and apical pulses.
(a) WT and (a′) sqh knockdown stage 7 follicles stained for F-actin (red) and FasIII (white). (b) Elongation coefficient of WT or sqh knockdown follicles during the early elongation phase (D, Driver;…
Figure 3—figure supplement 1
Myosin II is required for early elongation.
Follicles stained for pSqh (white in a,b, green in a’,b’) and F-actin (pink in a’b’). (a) WT follicle. (b) sqhRNAi in follicle cells driven with Tj:Gal4. The signal is mainly apical in WT follicle …
Figure 4 with 1 supplement
JAK-STAT induces a double gradient of pulses.
(a–d) Images from movies of the mediolateral region of (a) stage 3 and (c) stage 7 BAZ-GFP expressing follicles, or of the area near the polar cells (red arrowheads) of (b) stage 3 and (d) stage 7 …
Figure 4—figure supplement 1
Myosin II is required for early elongation.
(a) Mean percentage of apical surface variation in the mediolateral region relative to the follicle length (corresponding stages are also indicated). (b) Pulsing activity of individual cells from …
Figure 5
Myosin II is not controlled by JAK-STAT but is required at the poles.
(a) Apical level of phosphorylated Sqh (pSqh, white and [a′]) is reduced in a mutant Stat92E clone (RFP-negative) in a stage 3 follicle (z-projection of the superior half of the follicle). (b) …
Figure 6 with 1 supplement
Localized apical cell constriction and oriented cell intercalation occur during early elongation.
(a–c) AR and number of cells along the polar cell plane in representative (a) stage 6, (b) stage 7 and (c) stage 8 follicles stained for DE-Cad. (d–e) Number of follicular cells in the plane of …
Figure 6—figure supplement 1
No contribution of cell elongation and cell division to early elongation.
(a) Orientation of cells with elongated shape in the mediolateral part of the follicle at the indicated stages (the X-axis corresponds to the AP axis). (b) Magnitude of the elongated cell shape …
Author response image 1
Author response image 2
Videos
Video 1
Full z-stack of a follicle with a mys mutant clone that affects polar cells.
https://doi.org/10.7554/eLife.32943.004
Video 2
Full z-stack of a follicle with a mys mutant clone that does not affect polar cells.
https://doi.org/10.7554/eLife.32943.005
Video 3
Stage 3 follicle expressing Sqh-GFP.
The pool of apical myosinII is very dynamic.
Video 4
Zoom in on a cell of a stage 3 follicle expressing Baz-GFP and Sqh-mCherry.
Apical MyosinII enrichment occurs at the same time as the apical cell domain contracts.
Video 5
Stage 3 follicle expressing Baz-GFP.
Cells in the mediolateral part undergo apical pulsations.
Video 6
Stage 7 follicle expressing Baz-GFP.
The apical surface variation is strongly reduced on the mediolateral part compared with stage 3 follicles.
Video 7
Stage 7 follicle expressing Baz-GFP observed from the pole.
Polar cells are indicated on the corresponding Figure 5d (red arrowheads). The pulse intensity remains high in these cells compared with Video 4. The rotation is visible and occurs around the polar …
Video 8
Stage 3 follicle expressing Baz-GFP observed from the pole.
Polar cells are indicated on the corresponding Figure 5b (red arrowhead).
Video 9
Stage 3 upd knockdown follicle expressing Baz-GFP.
The intensity of the pulse is reduced compared with a WT stage 3 follicle (Video 3).
Video 10
Stage 7 upd knockdown follicle expressing Baz-GFP and observed from the pole.
The intensity of the pulse is reduced compared with a WT stage 7 follicle (Video 6).
Video 11
Stage 7 follicle expressing ectopically Baz-mCherry.
The intensity of the pulse is low.
Video 12
Stage 7 follicle expressing ectopically Baz-mCherry and Hoptum.
Activation of the JAK-STAT pathway is sufficient to increase the pulsing.
Video 13
Movie representing a stage 7 DE-Cad-GFP follicle imaged over two hours.
One cell is tracked (red line). No intercalation occurs during this period.
Additional files
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Supplementary file 1
Stock list and source.
The Baz-Cherry fusion protein was produced by cloning mCherry in frame at the C-terminus of the Par-6 coding sequence in the pUASP vector.
- https://doi.org/10.7554/eLife.32943.026
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Supplementary file 2
Detailed genotypes and specific conditions.
HS: 1 hr heat-shock at 37°C, when not specified flies were kept at 25°C.
- https://doi.org/10.7554/eLife.32943.027
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Supplementary file 3
Detailed sample size.
n corresponds to the number of analyzed follicles, with usually more than 10 segmented cells per follicle.
- https://doi.org/10.7554/eLife.32943.028
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Transparent reporting form
- https://doi.org/10.7554/eLife.32943.029
