Exportin Crm1 is repurposed as a docking protein to generate microtubule organizing centers at the nuclear pore
- University of Edinburgh, United Kingdom
- National Institute of Information and Communications Technology, Japan
Abstract
Non-centrosomal microtubule organizing centers (MTOCs) are important for microtubule organization in many cell types. In fission yeast Schizosaccharomyces pombe, the protein Mto1, together with partner protein Mto2 (Mto1/2 complex), recruits the g-tubulin complex to multiple non-centrosomal MTOCs, including the nuclear envelope (NE). Here, we develop a comparative-interactome mass spectrometry approach to determine how Mto1 localizes to the NE. Surprisingly, we find that Mto1, a constitutively cytoplasmic protein, docks at nuclear pore complexes (NPCs), via interaction with exportin Crm1 and cytoplasmic FG-nucleoporin Nup146. Although Mto1 is not a nuclear export cargo, it binds Crm1 via a nuclear export signal-like sequence, and docking requires both Ran in the GTP-bound state and Nup146 FG repeats. In addition to determining the mechanism of MTOC formation at the NE, our results reveal a novel role for Crm1 and the nuclear export machinery in the stable docking of a cytoplasmic protein complex at NPCs.
Data availability
The mass spectrometry proteomics data from both LFQ and SILAC experiments have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD008334
Article and author information
Author details
Funding
Wellcome (94517)
- Xun X Bao
- Eric M Lynch
- Kenneth E Sawin
Japan Society for the Promotion of Science (JP25116006)
- Tomoko Kojidani
- Tokuko Haraguchi
The Darwin Trust of Edinburgh
- Xun X Bao
Wellcome (108504)
- Christos Spanos
- Juri Rappsilber
Wellcome (91020)
- Christos Spanos
- Juri Rappsilber
Wellcome (203149)
- Xun X Bao
- Christos Spanos
- Eric M Lynch
- Juri Rappsilber
- Kenneth E Sawin
Japan Society for the Promotion of Science (JP17H03636)
- Tomoko Kojidani
- Tokuko Haraguchi
Japan Society for the Promotion of Science (JP17H01444)
- Yasushi Hiraoka
Japan Society for the Promotion of Science (JP16H01309)
- Yasushi Hiraoka
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2018, Bao et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
Metrics
-
- 2,430
- views
-
- 413
- downloads
-
- 19
- citations
Views, downloads and citations are aggregated across all versions of this paper published by eLife.
Citations by DOI
-
- 19
- citations for umbrella DOI https://doi.org/10.7554/eLife.33465
Download links
A two-part list of links to download the article, or parts of the article, in various formats.
Downloads (link to download the article as PDF)
Open citations (links to open the citations from this article in various online reference manager services)
Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)
Exportin Crm1 is repurposed as a docking protein to generate microtubule organizing centers at the nuclear pore
eLife 7:e33465.
https://doi.org/10.7554/eLife.33465
