(A) PomcCreERt2 mice were crossed with Ai14(RCL-tdT)-D mice, injected with tamoxifen using the described paradigm. Using immunohistochemistry, tdTomato expression (red) was colocalized with β-endorphin (marker of POMC neurons) expression (green) 2 weeks later. (B) The inducible Cre model does not recombine in extra-hypothalamic areas, except for a few β-endorphin + cells located in the nucleus tractus solitarius in the HB and the pituitary. (C) Leptin-induced Stat3 activation in the arcuate nucleus of the hypothalamus. Mice were injected with tamoxifen using the described paradigm and leptin (5 mg/kg, i.p.) was administrated in 16h-fasted animals. Animals were decapitated 45 min later and pStat3 was evaluated by immunohistochemistry. A representative image of n = 3 animals per group is shown. CB, cerebellum, CTX, cortex; DR, dorsal raphe; HB, hindbrain; HIP, hippocampus; OB, olfactory bulb; PT, pituitary; SC, spinal cord; STR, striatum.