(a) Upper panel: Schematic representation of infusions and OIC paradigm in rats. Lower panels: Discrimination indexes during test phases, 24 and 48 hrs after the training sessions. Lower left: Infusion of MDL in mPFC impairs discrimination between contextually congruent and incongruent objects during the test 1. ***p<0.0001, (n = 13–14 per group) unpaired Student's t test. Lower right: Infusion of EME in dCA1 immediately after test 1 affects memory retention evaluated 24 hrs later (test 2). Infusion of MDL in mPFC before test 1 did not modify the pattern of memories affected by EME. ***pinteraction <0.0001, (n = 6–7 per group), Two-way ANOVA followed by Bonferroni post-hoc test. (b) Upper panel: Schematic representation of infusions and behavioral paradigm. Rats were trained in the OIC paradigm but during test 1 were exposed to two copies of the contextually congruent object A. Immediately after test 1, rats were infused with VEH or EME in dCA1 and then exposed to the rest of the OIC task. Lower panel: Infusion of EME in dCA1 immediately after test 1 did not affect memory retention for any of the original objects (n = 5 per group). (c) mPFC 5-HT2aR blockade does not affect Zif268-dependent reconsolidation pattern of object memories in the dCA1. Upper panel: Schematic representation of infusions (ODNs in PRH and MDL/VEH in mPFC) and OIC paradigm. Lower panels: Discrimination indexes during test phases, 24 and 48 hrs after training sessions. Left: Discrimination index obtained during test 1 ***p<0.0001, (n = 14 per group), unpaired Student's t test. Right: Discrimination index obtained during test 2. *pinteraction = 0.03, ***p<0.0001, (n = 7 per group), Two-way ANOVA followed by Bonferroni post-hoc test. (d) Left: Representative spread of 1 nmol of biotinylated ODN, 90 min post injection in the dCA1. Right: Zif268-ASO injection decreases Zif268 protein levels. Top: representative Zif268 immunoblots of dCA1 protein extracts prepared 90 min after ODN infusion; actin was used as a loading control. Bottom: Quantification of Zif268 immunoblots. Actin-normalized Zif268 protein levels are shown, relative to the mean of the Zif268-MSO in the dCA1 of non-trained animals. **p=0.0019, (n = 5 per group), unpaired Student's t test. Data represent mean ±SEM. A and B represent objects presented in each session. During test sessions, A represents the contextually congruent and B the contextually incongruent objects.