Synapse-specific and compartmentalized expression of presynaptic homeostatic potentiation
- University of Southern California, United States
Figures
Figure 1 with 1 supplement
GluRIIA knock down phenocopies GluRIIA mutants.
(A) Representative images of NMJs on muscle six immunostained with antibodies that recognize GluRIIA, GluRIIC, and GluRIID receptor subunits in wild type (w1118), GluRIIA mutants (w1118;GluRIIASP16),…
Figure 1—figure supplement 1
Synaptic growth is mildly reduced at NMJs of GluRIIA mutants and G14 > GluRIIARNAi.
(A) Representative images of muscle 6/7 NMJs of wild type, GluRIIA mutants, and G14 > GluRIIARNAi immunostained with antibodies against with the neuronal membrane (HRP) and the synaptic vesicle …
Figure 2 with 1 supplement
GluRIIA-containing receptors can be knocked down specifically on muscle six using M6 >Gal4.
(A) Schematic of the Type Ib motor neuron innervating both muscles 6 and 7 at the Drosophila NMJ. Gal4 is expressed specifically on muscle six in combination with UAS-GluRIIARNAi using M6 > Gal4 (M6 …
Figure 2—figure supplement 1
Biased Gal-4 expression on muscle six does not alter synaptic growth or function.
(A) Representative images of muscle 6/7 NMJs of wild type and M6 > mCherryRNAi (w1118;Tub-FRT-STOP-FRT-Gal4, UAS-FLP, UAS-CD8-GFP/+; H94-Gal4, nSyb-Gal80/UAS-mCherryRNAi) labeled with phalloidin …
Figure 3
Presynaptic homeostatic potentiation can be induced and expressed exclusively at synapses innervating muscle 6.
(A) Schematic and electrophysiological traces of recordings from muscles 7 and 6 of wild type and M6 > GluRIIARNAi NMJs. Note that mEPSPs are reduced only on muscle 6 of M6 > GluRIIARNAi, while EPSP …
Figure 4
PHP can be induced and expressed acutely on muscle 7 following chronic GluRIIA knock down on M6.
(A) Schematic and representative traces illustrating the acute application of PhTx on wild type and M6 >GluRIIARNAi NMJs. The acute expression of PHP was observed on previously non-potentiated …
Figure 5
Homeostatic modulation of the readily releasable vesicle pool is restricted to synapses innervating muscle 6 at M6 >GluRIIARNAi NMJs.
(A) Schematic illustrating the size of the RRP is enhanced specifically at the terminals innervating muscle six in M6 > GluRIIARNAi. Representative traces of two-electrode voltage clamp recordings …
Figure 6
The number of functional release sites are specifically enhanced at synapses innervating muscle 6 at M6 >GluRIIARNAi NMJs.
(A) Schematic illustrating the number of functional release sites (marked as red triangles) at synapses innervating muscle 7 of M6 > GluRIIARNAi NMJs. (B) Scatter plot EPSC distribution of …
Figure 7
Compartmentalized changes in CaMKII activity at postsynaptic densities of M6 > GluRIIARNAi NMJs.
(A) Representative images of muscle 6/7 NMJs from wild type and M6 > GluRIIARNAi immunostained with antibodies against with the active (phosphorylated) form of CaMKII (pCaMKII) and the presynaptic …
Figure 8
Overexpression of constitutively active CaMKII inhibits retrograde PHP signaling at M6 > GluRIIARNAi NMJs.
(A) Schematic and representative traces illustrating that overexpression of a constitutively active form of CaMKII only on muscle 6 (M6>CaMKIIT287D) has no significant impact on neurotransmission. (B…
Additional files
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Supplementary file 1
Absolute values for all data and additional statistics.
The figure and panel, genotype, and conditions used are noted (muscle segment, external calcium concentration, PhTx application). All electrophysiological recordings were performed in 0.4 mM external calcium unless specified otherwise. Average values for mEPSP, EPSP, quantal content, resting potential, input resistance, number of data samples (n), p values, and significance are shown. Average values for confocal imaging intensity levels are also indicated. Standard error values are noted in parentheses.
- https://doi.org/10.7554/eLife.34338.013
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Transparent reporting form
- https://doi.org/10.7554/eLife.34338.014
