A stable mode of bookmarking by TBP recruits RNA Polymerase II to mitotic chromosomes

Abstract
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Abstract

Maintenance of transcription programs is challenged during mitosis when chromatin becomes condensed and transcription is silenced. How do the daughter cells re-establish the original transcription program? Here, we report that the TATA-binding protein (TBP), a key component of the core transcriptional machinery, remains bound globally to active promoters in mouse embryonic stem cells during mitosis. Using live-cell single-molecule imaging, we observed that TBP mitotic binding is highly stable, with an average residence time of minutes, in stark contrast to typical TFs with residence times of seconds. To test the functional effect of mitotic TBP binding, we used a drug-inducible degron system and found that TBP promotes the association of RNA Polymerase II with mitotic chromosomes, and facilitates transcriptional reactivation following mitosis. These results suggest that the core transcriptional machinery promotes efficient transcription maintenance globally.

Data availability

Sequencing data have been deposited in GEO under accession code GSE109964.

The following data sets were generated

1. Teves SS
(2018) A stable mode of bookmarking by TBP recruits RNAPolymerase II to mitotic chromosomes
Publicly available at the NCBI Gene Expression Omnibus (accession no: GSE109964).

https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE109964

The following previously published data sets were used

1. Tveves SS
2. Tjian R
(2016) Global accessibility of mitotic chromosomes
Publicly available at the NCBI Gene Expression Omnibus (accession no: GSE85184).

http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE85184

Article and author information

Author details

Sheila S Teves

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States

For correspondence
sheila.teves@berkeley.edu

Competing interests
No competing interests declared.

"This ORCID iD identifies the author of this article:" 0000-0002-1220-2414
Luye An

Department of Biochemistry, Molecular, and Cell Biology, Cornell University, Ithaca, United States

Competing interests
No competing interests declared.
Aarohi Bhargava-Shah

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States

Competing interests
No competing interests declared.
Liangqi Xie

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States

Competing interests
No competing interests declared.
Xavier Darzacq

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States

Competing interests
No competing interests declared.

"This ORCID iD identifies the author of this article:" 0000-0003-2537-8395
Robert Tjian

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States

For correspondence
jmlim@berkeley.edu

Competing interests
Robert Tjian, One of the three founding funders of eLife and a member of eLife's Board of Directors..

"This ORCID iD identifies the author of this article:" 0000-0003-0539-8217

Funding

Jane Coffin Childs Memorial Fund for Medical Research

Sheila S Teves

Howard Hughes Medical Institute

Robert Tjian

Siebel Stem Cell Institute

Sheila S Teves
Robert Tjian

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Copyright

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.