A stable mode of bookmarking by TBP recruits RNA Polymerase II to mitotic chromosomes
- University of California, Berkeley, United States
- Cornell University, United States
Abstract
Maintenance of transcription programs is challenged during mitosis when chromatin becomes condensed and transcription is silenced. How do the daughter cells re-establish the original transcription program? Here, we report that the TATA-binding protein (TBP), a key component of the core transcriptional machinery, remains bound globally to active promoters in mouse embryonic stem cells during mitosis. Using live-cell single-molecule imaging, we observed that TBP mitotic binding is highly stable, with an average residence time of minutes, in stark contrast to typical TFs with residence times of seconds. To test the functional effect of mitotic TBP binding, we used a drug-inducible degron system and found that TBP promotes the association of RNA Polymerase II with mitotic chromosomes, and facilitates transcriptional reactivation following mitosis. These results suggest that the core transcriptional machinery promotes efficient transcription maintenance globally.
Data availability
Sequencing data have been deposited in GEO under accession code GSE109964.
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A stable mode of bookmarking by TBP recruits RNAPolymerase II to mitotic chromosomesPublicly available at the NCBI Gene Expression Omnibus (accession no: GSE109964).
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Global accessibility of mitotic chromosomesPublicly available at the NCBI Gene Expression Omnibus (accession no: GSE85184).
Article and author information
Author details
Funding
Jane Coffin Childs Memorial Fund for Medical Research
- Sheila S Teves
Howard Hughes Medical Institute
- Robert Tjian
Siebel Stem Cell Institute
- Sheila S Teves
- Robert Tjian
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2018, Teves et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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A stable mode of bookmarking by TBP recruits RNA Polymerase II to mitotic chromosomes
eLife 7:e35621.
https://doi.org/10.7554/eLife.35621
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