(a) Schematic of Cre-dependent AAV-eGFP viral injection in ChAT-Cre or PV-Cre mice. (b) AAV-eGFP expression is highly colocalized with ChAT and PV immunostaining, respectively. Scale bars, 100 µm. …
(a) Schematic of Cre-dependent AAV eGFP injection in striatum of ChAT-Cre or PV-Cre mouse. (b) Quantification of ChAT immunostaining and eGFP expression colocalization. (c) Quantification of PV …
(a) Expression of TVA-mCherry positive starter cells, rabies-eGFP and immunostaining for PV in dorsal striatal injection site. Scale bar, 100 um. (b) Magnified inset of cortex (Ctx) and dorsal …
(a) Major input regions (>0.4% of total inputs) to ChAT (black bar) and PV (red bar) interneurons. A complete set of all inputs to ChAT and PV interneurons is shown in Figure 2—figure supplement 1. …
(a) All major (>0.4%) and minor (<0.4%) inputs to striatal ChAT (black) and PV (red) interneurons. Short dashed line represents division between major and minor inputs. Mean ± SEM. In blue, *p≤0.05; …
Striatal ChAT interneurons receive preferential inputs from orbital cortex, retrosplenial cortex, intralaminar thalamic nuclei, mediodorsal thalamus and the pedunculopontine nucleus and might be …
(a) PV interneurons receive a thalamic reticular nucleus (TRN) projection. (b) Rabies-eGFP positive neurons in TRN colocalize with PV immunostaining. Scale bar, 50 µm. Thal = thalamus. (c) …
Rabies positive eGFP cells in TRN colocalize with parvalbumin and somatostatin immunostaining. Somatostatin (Som) positive TRN neurons send projections to striatum. (a–d) Capacitance, membrane …
(a) Cartoon schematic of multichannel array placement with optical fiber for light delivery in dorsal striatum. (b) Grayscale DAPI stained images of striatal slices (left) following in vivo …
(a) 3D plot of all recorded units by peak half-width (ms), amplitude (mV) and firing rate (Hz). Putative PV interneurons sorted by narrow half widths (<0.12 ms). Red circles denotes putative fast …
(a) Experimental approach to label GPe inputs with ChR2 and express tdTomato in ChAT or PV interneurons. (b) Averaged paired-pulse (50 ms ISI) light-evoked IPSCs in striatal ChAT interneuron …
(a) GPe is the predominant basal ganglia input to ChAT and PV interneurons (two-way ANOVA, F (6,63)=27.27, p<0.0001). (b–c) (top) Few GPe rabies-eGFP neurons colocalize with immunostaining for PV, …
(a) Cartoon schematic showing whole-cell recordings from pairs of interneurons and neighboring SPNs following light-evoked GPe terminal stimulation. (b) IPSCs from pairs of tdTomato (+) ChAT …
(a) Rabies-labeled projections to striatal ChAT interneurons from the PPN are not cholinergic. Note no colocalized eGFP (green) and ChAT immunostaining (red). scp, superior cerebellar peduncle. …
Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Strain, strain background (Mus musculus) | ChAT-IRES-Cre | Jackson Labs | stock #006410; RRID:IMSR_JAX:006410 | maintained on a C57BL6/J background |
Strain, strain background (Mus musculus) | PV-Cre | Jackson Labs | stock #008069; RRID:IMSR_JAX:008069 | maintained on a C57BL6/J background |
Strain, strain background (Mus musculus) | Sst-IRES-Cre | Jackson Labs | stock #028864; RRID:IMSR_JAX:028864 | maintained on a C57BL6/J background |
Strain, strain background (Adeno-associated virus) | AAV5-TVA-mCherry | UNC Viral Vector Core | RRID: SCR_002448 | 3–4.3 × 1012 particles/mL |
Strain, strain background (Adeno-associated virus) | AAV8-CA-RG | UNC Viral Vector Core | RRID: SCR_002448 | 1.2–4.3 × 1012 particles/mL |
Strain, strain background (Adeno-associated virus) | (EnvA) SAD-∆G Rabies-eGFP | Salk Vector Core | RRID: SCR_014847 | 1.6–6.55 × 108 particles/mL |
Strain, strain background (Adeno-associated virus) | AAV9-Ef1a-DIO-ChR2 (H134R)-eYFP | University of Penn Viral Vector Core | RRID: SCR_015406 | two × 1012 particles/mL |
Strain, strain background (Adeno-associated virus) | AAV9-FLEX-tdTomato | University of Penn Viral Vector Core | RRID: SCR_015406 | two × 1012 particles/mL |
Strain, strain background (Adeno-associated virus) | AAVretro-EF1a-Flp | UNC Viral Vector Core | RRID: SCR_002448 | two × 1012 particles/mL |
Strain, strain background (Adeno-associated virus) | AAV9-hsyn-Con-Fon-hChR2-eYFP | UNC Viral Vector Core | RRID: SCR_002448 | two × 1012 particles/mL |
Strain, strain background (Adeno-associated virus) | AAV9-FLEX-eGFP | University of Penn Viral Vector Core | RRID: SCR_015406 | two × 1012 particles/mL |
Strain, strain background (Adeno-associated virus) | AAV9-hsyn-ChR2-eYFP | University of Penn Viral Vector Core | RRID: SCR_015406 | two × 1012 particles/mL |
Antibody | anti-PV (mouse,monoclonal) | MilliporeSigma | P3088; RRID: AB_477329 | 1/1000 |
Antibody | anti-PV (rabbit, polyclonal) | Abcam | ab11427; RRID: AB_298032 | 1/1000 |
Antibody | anti-ChAT (goat, polyclonal) | MilliporeSigma | AB144P; RRID: AB_2079751 | 1/100 |
Antibody | anti-NPAS1 (rabbit, polyclonal) | GeneTex | GTX105876; RRID: AB_424768 | 1/500 |
Antibody | anti-mCherry (mouse, monoclonal) | Takara Bio USA, Inc (Clontech Labs) | 632543; RRID: AB_2307319 | 1/250 |
Antibody | anti-eGFP (rabbit, polyclonal) | Thermo Fisher Scientific | A11122; RRID: AB_1074875 | 1/400 |
Antibody | anti-somatostatin (rabbit, polyclonal) | Protos Biotech Corp | NP106SST | 1/300 |
Antibody | anti-FoxP2 (rabbit, polyclonal) | Sigma | HPA000382; RRID: AB_1078908 | 1/1000 |
Antibody | donkey anti-mouse 488 (secondary) | Jackson Immunoresearch | 715-545-150; RRID: AB_2340846 | 1/250 |
Antibody | donkey anti-mouse CY3 (secondary) | Jackson Immunoresearch | 715-165-150; RRID: AB_2340813 | 1/250 |
Antibody | donkey anti-mouse CY5 (secondary) | Jackson Immunoresearch | 715-175-150; RRID: AB_2340819 | 1/250 |
Antibody | donkey anti-rabbit 488 (secondary) | Jackson Immunoresearch | 711-545-152; RRID: AB_2313584 | 1/250 |
Antibody | donkey anti-rabbit CY3 (secondary) | Jackson Immunoresearch | 711-165-152; RRID: AB_2307443 | 1/250 |
Antibody | donkey anti-rabbit CY5 (secondary) | Jackson Immunoresearch | 711-175-152; RRID: AB_2340607 | 1/250 |
Antibody | donkey anti-goat 488 (secondary) | Jackson Immunoresearch | 705-545-147; RRID: AB_2336933 | 1/250 |
Antibody | donkey anti-goat CY3 (secondary) | Jackson Immunoresearch | 705-165-147; RRID: AB_2307351 | 1/250 |
Antibody | donkey anti-goat CY5 (secondary) | Jackson Immunoresearch | 705-175-147; RRID: AB_2340415 | 1/250 |
Chemical compound, drug | NBQX disodium salt hydrate | MilliporeSigma | N183 | 10 uM (final) |
Chemical compound, drug | DL-APV | MilliporeSigma | A5282 | 50 uM (final) |
Chemical compound, drug | Picrotoxin | MilliporeSigma | P1675 | 50–100 uM (final) |
Chemical compound, drug | NMDG | MilliporeSigma | M2004 | |
Chemical compound, drug | HCl | MilliporeSigma | H1758 | |
Chemical compound, drug | KCl | MilliporeSigma | P9541 | |
Chemical compound, drug | NaH2PO4 | MilliporeSigma | S3139 | |
Chemical compound, drug | NaHCO3 | MilliporeSigma | S6014 | |
Chemical compound, drug | Glucose | MilliporeSigma | G5767 | |
Chemical compound, drug | Sodium L-Ascorbate | MilliporeSigma | A4034 | |
Chemical compound, drug | Sodium Pyruvate | MilliporeSigma | P2256 | |
Chemical compound, drug | Thiourea | MilliporeSigma | T8656 | |
Chemical compound, drug | MgSO4 | MilliporeSigma | M2643 | |
Chemical compound, drug | CaCl2 | MilliporeSigma | 223506 | |
Chemical compound, drug | MgCl2 | MilliporeSigma | M9272 | |
Chemical compound, drug | KMeSO4 | MilliporeSigma | 83000 | |
Chemical compound, drug | HEPES | MilliporeSigma | H4034 | |
Chemical compound, drug | EGTA | MilliporeSigma | 3777 | |
Chemical compound, drug | Mg-ATP | MilliporeSigma | A9187 | |
Chemical compound, drug | Na-GTP | MilliporeSigma | G8877 | |
Chemical compound, drug | CsCl | MilliporeSigma | C4036 | |
Chemical compound, drug | CsMeSO3 | MilliporeSigma | C1426 | |
Chemical compound, drug | QX-314 | MilliporeSigma | L5783 | |
Chemical compound, drug | TEA-Cl | MilliporeSigma | T2265 | |
Software, algorithm | MATLAB | RRID: SCR_001622 | ||
Software, algorithm | GraphPad Prism 6 | RRID: SCR_002798 | ||
Software, algorithm | Adobe Illustrator CS6 | RRID: SCR_010279 | ||
Software, algorithm | pClamp9 | RRID: SCR_011323 | ||
Software, algorithm | Fiji/Imagej | RRID: SCR_002285 | ||
Other | Allen Reference Atlas | RRID: SCR_013286 |
MATLAB script for perievent time histogram (PETH) plot.
Plot PETH of ChAT or PV interneuron firing rates aligned to laser on (time zero).