(A, B) Representative EPSC traces recorded before (black) and 30 min after (blue) the conditioning stimulation in the H zone of untrained (A) or trained (B) mice. (C, D) Time courses of normalized EPSC amplitudes before and after the conditioning stimulation at PF-PC synapse in the H zone (untrained, open circles, n = 14; trained, filled circles, n = 12) (C) or the paraflocculus (untrained, open circles, n = 6; trained, filled circles, n = 7) (D). EPSC amplitudes were normalized by setting the amplitude between −1 min and 0 min at 100%. At 0 min, the conditioning depolarizations coupled with the PF stimulation were applied to a PC. (E, F) Distribution of EPSC amplitudes at 30 min after the start of conditioning relative to those before in the H zone (E) and in the paraflocculus (F). Each small circle represents a result obtained from a PC (red, blind experiment; black, non-blind experiment), and large circles and bars represent Mean ± SEM of all data. ***p<0.001, Student’s t-test at 30 min.