Inhibition gates supralinear Ca2+ signaling in Purkinje cell dendrites during practiced movements
- Max Planck Florida Institute for Neuroscience, United States
- Gunma University, Japan
Abstract
Motor learning involves neural circuit modifications in the cerebellar cortex, likely through re-weighting of parallel fiber inputs onto Purkinje cells (PCs). Climbing fibers instruct these synaptic modifications when they excite PCs in conjunction with parallel fiber activity, a pairing that enhances climbing fiber-evoked Ca2+ signaling in PC dendrites. In vivo, climbing fibers spike continuously, including during movements when parallel fibers are simultaneously conveying sensorimotor information to PCs. Whether parallel fiber activity enhances climbing fiber Ca2+ signaling during motor behaviors is unknown. In mice, we found that inhibitory molecular layer interneurons (MLIs), activated by parallel fibers during practiced movements, suppressed parallel fiber enhancement of climbing fiber Ca2+ signaling in PCs. Similar results were obtained in acute slices for brief parallel fiber stimuli. Interestingly, more prolonged parallel fiber excitation revealed latent supralinear Ca2+ signaling. Therefore, the balance of parallel fiber and MLI input onto PCs regulates concomitant climbing fiber Ca2+ signaling.
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All data are included in the manuscript or the source data files.
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Author details
Funding
National Institutes of Health (NS083894)
- Jason Christie
Max Planck Society
- Jason Christie
Max Planck Florida Institute for Neuroscience
- Jason Christie
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Ethics
Animal experimentation: Animal procedures were conducted using protocol 15-205 approved by the Institutional Animal Care and Use Committee (IACUC) at Max Planck Florida Institute for Neuroscience.
Copyright
© 2018, Gaffield et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Inhibition gates supralinear Ca2+ signaling in Purkinje cell dendrites during practiced movements
eLife 7:e36246.
https://doi.org/10.7554/eLife.36246
