31P NMR spectra of 20 mM 2-AIpG after 3 min (A) or 3 hr (B) of incubation in crystal soaking buffer (5% v/v (+/-) −2-methyl-2, 4-pentanediol, 20 mM sodium cacodylate pH 7.0, 6 mM spermine tetrahydrocholoride, 40 mM sodium chloride) with 20 mM MgCl2. The large peak at −11.11 ppm corresponds to 2-AIpG. During the three hours of incubation, only 7.8% of the monomer decays, mostly to an unknown product corresponding to the peak at 5.99 ppm that may represent reaction with a buffer component. The small peak at −12.92 ppm corresponds to Gp-AI-pG. The experiment was performed in triplicate.