Indirect calorimetric assessments following saline or CNO injection for the 24 hr time course of energy expenditure (A, B, C), physical activity represented as total count (D, E, F), and respiratory exchange ratio (G, H, I) in Cartptcre/cre (A, D, G), Cartptcre/+ (B, E, H) without hM3Dq-injection and wild type mice receiving bilateral LHA→hM3Dq but lacking Cre-recombinase expression (C, F, I), respectively. Open and filled horizontal bars indicate the light and dark photoperiods, respectively. Dotted lines indicate the time of i.p. injection of saline or CNO. Data are means ±SEM. n = 8.