In vivo detection of optically-evoked opioid peptide release

  1. Ream Al-Hasani  Is a corresponding author
  2. Jenny-Marie T Wong
  3. Omar S Mabrouk
  4. Jordan G McCall
  5. Gavin P Schmitz
  6. Kirsten A Porter-Stransky
  7. Brandon J Aragona
  8. Robert T Kennedy  Is a corresponding author
  9. Michael R Bruchas  Is a corresponding author
  1. Washington University School of Medicine, United States
  2. University of Michigan, United States

Abstract

Though the last decade has seen accelerated advances in techniques and technologies to perturb neuronal circuitry in the brain, we are still poorly equipped to adequately dissect endogenous peptide release in vivo. To this end we developed a system that combines in vivo optogenetics with microdialysis and a highly sensitive mass spectrometry-based assay to measure opioid peptide release in freely moving rodents.

Data availability

All data generated or analysed during this study are included in the manuscript and supporting files

Article and author information

Author details

  1. Ream Al-Hasani

    Department of Anesthesiology, Division of Basic Research, Washington University School of Medicine, St Louis, United States
    For correspondence
    al-hasanir@wustl.edu
    Competing interests
    No competing interests declared.
  2. Jenny-Marie T Wong

    Department of Chemistry, University of Michigan, Ann Arbor, United States
    Competing interests
    No competing interests declared.
  3. Omar S Mabrouk

    Department of Chemistry, University of Michigan, Ann Arbor, United States
    Competing interests
    No competing interests declared.
  4. Jordan G McCall

    Department of Anesthesiology, Division of Basic Research, Washington University School of Medicine, St Louis, United States
    Competing interests
    No competing interests declared.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-8295-0664
  5. Gavin P Schmitz

    Department of Anesthesiology, Division of Basic Research, Washington University School of Medicine, St Louis, United States
    Competing interests
    No competing interests declared.
  6. Kirsten A Porter-Stransky

    Department of Psychology, University of Michigan, Ann Arbor, United States
    Competing interests
    No competing interests declared.
  7. Brandon J Aragona

    Department of Psychology, University of Michigan, Ann Arbor, United States
    Competing interests
    No competing interests declared.
  8. Robert T Kennedy

    Department of Chemistry, University of Michigan, Ann Arbor, United States
    For correspondence
    rtkenn@umich.edu
    Competing interests
    No competing interests declared.
  9. Michael R Bruchas

    Department of Anesthesiology, Division of Basic Research, Washington University School of Medicine, St Louis, United States
    For correspondence
    bruchasm@wustl.edu
    Competing interests
    Michael R Bruchas, Co-founder of Neurolux, Inc, a company that is making wireless optogenetic probes. None of the work in this manuscript used these devices or is related to any of the company's activities, but we list this information here in full disclosure.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-4713-7816

Funding

National Institute on Drug Abuse (K99/R00 Pathway to Independence DA038725)

  • Ream Al-Hasani

National Institute of Mental Health (F31 MH101956)

  • Jordan G McCall

National Institute of Biomedical Imaging and Bioengineering (R01 EB003320)

  • Robert T Kennedy

National Institute on Drug Abuse (R01 DA033396)

  • Michael R Bruchas

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Julie A Kauer, Brown University, United States

Ethics

Animal experimentation: This study was performed in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. All of the animals were handled according to approved institutional animal care and use committee (IACUC) protocols of Washington University in St. Louis and the University of Michigan.

Version history

  1. Received: March 9, 2018
  2. Accepted: September 2, 2018
  3. Accepted Manuscript published: September 3, 2018 (version 1)
  4. Version of Record published: September 12, 2018 (version 2)

Copyright

© 2018, Al-Hasani et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Ream Al-Hasani
  2. Jenny-Marie T Wong
  3. Omar S Mabrouk
  4. Jordan G McCall
  5. Gavin P Schmitz
  6. Kirsten A Porter-Stransky
  7. Brandon J Aragona
  8. Robert T Kennedy
  9. Michael R Bruchas
(2018)
In vivo detection of optically-evoked opioid peptide release
eLife 7:e36520.
https://doi.org/10.7554/eLife.36520

Share this article

https://doi.org/10.7554/eLife.36520

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