In vivo detection of optically-evoked opioid peptide release
- Washington University School of Medicine, United States
- University of Michigan, United States
Abstract
Though the last decade has seen accelerated advances in techniques and technologies to perturb neuronal circuitry in the brain, we are still poorly equipped to adequately dissect endogenous peptide release in vivo. To this end we developed a system that combines in vivo optogenetics with microdialysis and a highly sensitive mass spectrometry-based assay to measure opioid peptide release in freely moving rodents.
Data availability
All data generated or analysed during this study are included in the manuscript and supporting files
Article and author information
Author details
Funding
National Institute on Drug Abuse (K99/R00 Pathway to Independence DA038725)
- Ream Al-Hasani
National Institute of Mental Health (F31 MH101956)
- Jordan G McCall
National Institute of Biomedical Imaging and Bioengineering (R01 EB003320)
- Robert T Kennedy
National Institute on Drug Abuse (R01 DA033396)
- Michael R Bruchas
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Julie A Kauer, Brown University, United States
Ethics
Animal experimentation: This study was performed in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. All of the animals were handled according to approved institutional animal care and use committee (IACUC) protocols of Washington University in St. Louis and the University of Michigan.
Version history
- Received: March 9, 2018
- Accepted: September 2, 2018
- Accepted Manuscript published: September 3, 2018 (version 1)
- Version of Record published: September 12, 2018 (version 2)
Copyright
© 2018, Al-Hasani et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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In vivo detection of optically-evoked opioid peptide release
eLife 7:e36520.
https://doi.org/10.7554/eLife.36520
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