Flow data (FCS files) used to quantify fluorescence levels produced by the 43 TDH3 promoter alleles are available in the FlowRepository (flowrepository.org) under experiment ID FR-FCM-ZY8Y. Raw bright field and fluorescence images, as well as bright field images where cell division events were annotated, are available on Zenodo (https://zenodo.org) with DOI 10.5281/zenodo.1327545. All other data are provided as source data and/or supplementary files with the manuscript.
Time-lapse images of yeast cells with different levels of TDH3 median expression and expression noisePublicly available at Zenodo (https://zenodo.org/).
Activity of 48 TDH3 promoter variants in YPDPublicly available at FlowRepository (accession no. FR-FCM-ZY8Y).
Effects of 20 TDH3 promoter alleles at HO and native TDH3 lociPublicly available at FlowRepository (accession no. FR-FCM-ZYJX).
Fitness of 48 TDH3 promoter alleles in YPDPublicly available at FlowRepository (accession no. FR-FCM-ZYJN).
Supplementary file 1
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Source data 1
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