VP2, VP6 and VP7 are depicted in green, blue and yellow colors, respectively. Regarding the nominal height of each particle (55 nm for SLP, 70 nm for DLP and 76 nm for TLP), the SLP suffers the lowest percentage of deformation (2%) after substrate adsorption. The substrate deforms the DLP by 6%. The TLP without spikes at the interface between the particle and the substrate (62 nm) undergoes the highest percentage of deformation (18%), suggesting a strong interaction of the VP7 layer and the HOPG. This high deformation is hugely reduced to 3% in the TLP with spikes present at the particle-substrate interface (74 nm).