A resource for the Drosophila antennal lobe provided by the connectome of glomerulus VA1v
- Dalhousie University, Canada
- Janelia Research Campus, Howard Hughes Medical Institute, United States
Abstract
Using FIB-SEM we report the entire synaptic connectome of glomerulus VA1v of the right antennal lobe in Drosophila melanogaster. Within the glomerulus we densely reconstructed all neurons, including hitherto elusive local interneurons. The fruitless-positive, sexually dimorphic VA1v included >11,140 presynaptic sites with ~38,050 postsynaptic dendrites. These connected input olfactory receptor neurons (ORNs, 51 ipsilateral, 56 contralateral), output projection neurons (18 PNs), and local interneurons (56 of >150 previously reported LNs). ORNs are predominantly presynaptic and PNs predominantly postsynaptic; newly reported LN circuits are largely an equal mixture and confer extensive synaptic reciprocity, except the newly reported LN2V with input from ORNs and outputs mostly to monoglomerular PNs, however. PNs were more numerous than previously reported from genetic screens, suggesting that the latter failed to reach saturation. We report a matrix of 192 bodies each having >50 connections; these form 88% of the glomerulus' pre/postsynaptic sites.
Data availability
All data generated or analysed during this study are included in the manuscript and supporting files. Source data files have been provided for Figures 5, 8 and Figure 2-source data 1. Grayscale and segmentation data are hosted at a Janelia website: http://emdata.janelia.org/AL-VA1v. Data can be viewed in a web browser using neuroglancer. Please see the readme file on how to access the data programmatically using dvid and DICED (this can be accessed by clicking on ""AL-VA1v"" (hyperlinked) at http://emdata.janelia.org/AL-VA1v).
Article and author information
Author details
Ian A Meinertzhagen
Funding
Howard Hughes Medical Institute (Janelia FlyEM)
- Jane Anne Horne
- Carlie Langille
- Sari McLin
- Meagan Wiederman
- Zhiyuan Lu
- C Shan Xu
- Stephen M Plaza
- Louis K Scheffer
- Harald F Hess
- Ian A Meinertzhagen
The funder (HHMI) provided technical support for study design, and data collection.
Reviewing Editor
- Liqun Luo, Howard Hughes Medical Institute, Stanford University, United States
Publication history
- Received: April 14, 2018
- Accepted: October 31, 2018
- Accepted Manuscript published: November 1, 2018 (version 1)
- Version of Record published: November 13, 2018 (version 2)
Copyright
© 2018, Horne et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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A resource for the Drosophila antennal lobe provided by the connectome of glomerulus VA1v
eLife 7:e37550.
https://doi.org/10.7554/eLife.37550
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