Par3 interacts with Prickle3 to generate apical PCP complexes in the vertebrate neural plate

  1. Ilya Chuykin
  2. Olga Ossipova
  3. Sergei Y Sokol  Is a corresponding author
  1. Icahn School of Medicine at Mount Sinai, United States

Abstract

Vertebrate neural tube formation depends on the coordinated orientation of cells in the tissue known as planar cell polarity (PCP). In the Xenopus neural plate, PCP is marked by the enrichment of the conserved proteins Prickle3 and Vangl2 at anterior cell boundaries. Here we show that the apical determinant Par3 is also planar polarized in the neuroepithelium, suggesting a role for Par3 in PCP. Consistent with this hypothesis, interference with Par3 activity inhibited asymmetric distribution of PCP junctional complexes and caused neural tube defects. Importantly, Par3 physically associated with Prickle3 and promoted its apical localization, whereas overexpression of a Prickle3-binding Par3 fragment disrupted PCP in the neural plate. We also adapted proximity biotinylation assay for use in Xenopus embryos and show that Par3 functions by enhancing the formation of the anterior apical PCP complex. These findings describe a mechanistic link between the apical localization of PCP components and morphogenetic movements underlying neurulation.

Data availability

All data generated or analyzed during this study are included in the manuscript and supporting files.

Article and author information

Author details

  1. Ilya Chuykin

    Department of Cell, Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, United States
    Competing interests
    The authors declare that no competing interests exist.
  2. Olga Ossipova

    Department of Cell, Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, United States
    Competing interests
    The authors declare that no competing interests exist.
  3. Sergei Y Sokol

    Department of Cell, Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, United States
    For correspondence
    sergei.sokol@mssm.edu
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-3963-9202

Funding

National Institutes of Health (GM122492)

  • Sergei Y Sokol

National Institutes of Health (NS100759)

  • Sergei Y Sokol

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Ethics

Animal experimentation: This study was carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. The protocol 04-1295 was approved by the IACUC of the Icahn School of Medicine at Mount Sinai.

Reviewing Editor

  1. Yukiko M Yamashita, University of Michigan, United States

Publication history

  1. Received: April 25, 2018
  2. Accepted: September 25, 2018
  3. Accepted Manuscript published: September 26, 2018 (version 1)
  4. Version of Record published: October 8, 2018 (version 2)

Copyright

© 2018, Chuykin et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Ilya Chuykin
  2. Olga Ossipova
  3. Sergei Y Sokol
(2018)
Par3 interacts with Prickle3 to generate apical PCP complexes in the vertebrate neural plate
eLife 7:e37881.
https://doi.org/10.7554/eLife.37881

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