A multicellular rosette-mediated collective dendrite extension
- Sloan Kettering Institute, United States
- Boston Children’s Hospital, United States
- Harvard Medical School, United States
Figures
Figure 1 with 1 supplement
Multicellular rosette precedes collective dendrite extension.
(A, B) Amphid neurons form a multicellular rosette. (A) Image of embryo prior to amphid dendrite extension stained with phalloidin. A multicellular rosette is shaded in pink. In this and all …
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Figure 1—source data 1
Displacement of dendrite tip from five embryos.
- https://doi.org/10.7554/eLife.38065.004
Figure 1—figure supplement 1
Retrograde extension in wild-type embryos.
After the initial anterior extension is complete and the dendrite tips reach the sensory depression (top panel), cell bodies start to move posteriorly as the embryo elongates, further extending the …
Figure 2
The amphid rosette vertex is attached to the epidermis.
(A) PAR-6-labeled amphid tip (arrows) is localized at the hyp5 leading edge. The apical junction of epidermal cells was labeled by DLG-1::GFP with xnIs17 transgene. The inset is shown magnified on …
Figure 3 with 1 supplement
Multiple adhesion molecules function redundantly in amphid dendrite anterior extension.
(A–D) Localization of DYF-7, HMR-1, SAX-7 and DLG-1 (dlg-1(cp301)) in amphid neurons and epidermal cells. Middle panels, superficial focal plane showing localization in epidermal cells. Lower …
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Figure 3—source data 1
P values in multiple comparasions.
- https://doi.org/10.7554/eLife.38065.005
Figure 3—figure supplement 1
dyf-7 phenotype and genetic redundancy.
(A) Retrograde extension defects in dyf-7(m537) embryos.The dendrite tips detach from the sensory depression during embryo elongation, after successful anterior extension. See Figure 1—figure …
Figure 4 with 1 supplement
PAR-6 regulates localization of DYF-7.
(A, B) DYF-7 shows more dispersed localization in par-6(M/Z). (C) Quantification of the size of DYF-7::GFP in WT and par-6(M/Z) embryos before the dendrite tips migrate anteriorly. Data was shown as …
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Figure 4—source data 1
Size of DYF-7::GFP in wt and par-6(M/Z) embryos.
- https://doi.org/10.7554/eLife.38065.006
Figure 4—figure supplement 1
PAR-6 is required for amphid dendrite extension.
(A) The wild type. (B) Partial extension of amphid dendrites in par-6(M/Z) embryos that arrested at the 1.5-fold stage, indicating abnormal epidermal morphogenesis. (C) Partial extension of amphid …
Videos
Video 1
Amphid dendrite anterior extension.
Time-lapse imaging of a wild-type embryo expressing cnd-1p::PH::mCherry to label sensory neurons and PAR-6::GFP to label dendrite tips. Dashed lines mark the initial position of the amphid neuron …
Tables
Key resources table
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (E. coli) | OP50 | Caenorhabditis Genetics Center | OP50 | |
| Genetic reagent (C. elegans) | dex-1(ns42) III | PMID: 19344940 | ||
| Genetic reagent (C. elegans) | dyf-7 (ns119) X | PMID: 19344940 | ||
| Genetic reagent (C. elegans) | dyf-7 (m537) X | PMID: 19344940 | ||
| Genetic reagent (C. elegans) | sax-7(ky146) IV | Caenorhabditis Genetics Center | CX2993 | |
| Genetic reagent (C. elegans) | sax-7(eq1) IV | Caenorhabditis Genetics Center | LH81 | |
| Genetic reagent (C. elegans) | par-6(zu170) I | Caenorhabditis Genetics Center | FT36 | |
| Genetic reagent (C. elegans) | par-6(tm1425)/hIn1 [unc-54(h1040)] I | Caenorhabditis Genetics Center | JJ1743 | |
| Genetic reagent (C. elegans) | unc-101(m1) I | Caenorhabditis Genetics Center | DR1 | |
| Genetic reagent (C. elegans) | zbIs3[cnd-1p::PH::GFP] | PMID: 28441532 | ||
| Genetic reagent (C. elegans) | ujIs113[pie-1p::mCherry::H2B; nhr-2p::mCherry::HIS-24 -let-858UTR; unc-119(+)] II | Caenorhabditis Genetics Center | JIM113 | |
| Genetic reagent (C. elegans) | itIs1024[par-6::PAR-6::GFP] | Dr. Kenneth Kemphues | KK1024 | |
| Genetic reagent (C. elegans) | zyIs36[cnd-1p::PH::mCherry; myo-2p::mCherry]X | PMID: 28441532 | ||
| Genetic reagent (C. elegans) | mcIs40 [lin-26p::ABDvab- 10::mCherry; myo-2p::GFP] | Caenorhabditis Genetics Center | ML916 | |
| Genetic reagent (C. elegans) | xnIs17[dlg-1::GFP; rol-6(su1006)] | Caenorhabditis Genetics Center | FT63 | |
| Genetic reagent (C. elegans) | axIs1928[mCherry::PAR-6] | Caenorhabditis Genetics Center | JH2648 | |
| Genetic reagent (C. elegans) | dlg-1(cp301[dlg-1:: mNG-C1^3xFlag])X | Caenorhabditis Genetics Center | LP598 | |
| Genetic reagent (C. elegans) | ntIs1[gcy-5::GFP] | Caenorhabditis Genetics Center | OH3192 | |
| Genetic reagent (C. elegans) | oyIs44[odr-1::RFP + lin-15(+)]V | Caenorhabditis Genetics Center | PY2417 | |
| Genetic reagent (C. elegans) | zuIs43[pie-1::GFP::PAR-6::ZF1; unc-119(+)] | Caenorhabditis Genetics Center | JJ1743 | |
| Genetic reagent (C. elegans) | ddIs290[sax-7::TY1::EGFP:: 3xFLAG(92C12); unc-119(+)] | Caenorhabditis Genetics Center | TH502 | |
| Genetic reagent (C. elegans) | xnIs96 [hmr-1p::HMR-1::GFP:: unc-54 3'UTR; unc-119(+)] | Caenorhabditis Genetics Center | FT250 | |
| Genetic reagent (C. elegans) | kyIs4[ceh-23-unc-76-gfp::lin-15]X | Caenorhabditis Genetics Center | CX2565 | |
| Genetic reagent (C. elegans) | hmnEx149[dyf-7p::DYF-7 (ZP-sfGFP)-mCherry; rol-6(su1006)] | DOI: 10.1101/393850 | ||
| Genetic reagent (E. coli) | elt-1(RNAi) expressed in HT115 (DE3) | Source BioScience | C. elegans RNAi collection (Vadel) | RNAi Bacteria, Clone ID: 10019-B-8 |
| Genetic reagent (E. coli) | hmr-1(RNAi) expressed in HT115 (DE3) | Source BioScience | C. elegans RNAi collection (Ahringer) | RNAi Bacteria, Clone ID: I-5F23 |
| Genetic reagent (E. coli) | dlg-1(RNAi) expressed in HT115 (DE3) | Source BioScience | C. elegans RNAi collection (Ahringer) | RNAi Bacteria, Clone ID: X-8A08 |
| Software, algorithm | Fiji | https://fiji.sc/ |
Additional files
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Transparent reporting form
- https://doi.org/10.7554/eLife.38065.013
