1. Cell Biology

Comment on ‘Orthogonal lipid sensors identify transbilayer asymmetry of plasma membrane cholesterol’

  1. Kevin C Courtney  Is a corresponding author
  2. Karen YY Fung
  3. Frederick R Maxfield  Is a corresponding author
  4. Gregory D Fairn  Is a corresponding author
  5. Xiaohui Zha  Is a corresponding author
  1. University of Ottawa, Canada
  2. St. Michael’s Hospital, Canada
  3. Weill Cornell Medical College, United States
  4. Ottawa Hospital Research Institute, Canada
https://doi.org/10.7554/eLife.38493
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  1. Kevin C Courtney
  2. Karen YY Fung
  3. Frederick R Maxfield
  4. Gregory D Fairn
  5. Xiaohui Zha
(2018)
Comment on ‘Orthogonal lipid sensors identify transbilayer asymmetry of plasma membrane cholesterol’
eLife 7:e38493.
https://doi.org/10.7554/eLife.38493
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2 figures and 1 additional file

Figures

Figure 1
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D4 binding is influenced by phospholipid composition and is subject to competition from proteins.

(A) Purified DAN-D4 (0.5 µM) was incubated with 100 µM large unilamellar vesicles (LUVs) composed of POPC/egg SM/cholesterol (36:24:40) and POPC/POPE/POPS/soy PI/cholesterol (18:18:18:6:40). The …

https://doi.org/10.7554/eLife.38493.002
Figure 2
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D4 variants can bind to the cytoplasmic leaflet of the PM in a cholesterol-dependent manner.

(A) CHO cells transiently transfected with mCherry-D4D434A and D4D434A, A463W and the plasma membrane marker, Pleckstrin homology domain of phospholipase C δ (PH-PLC δ) were examined using …

https://doi.org/10.7554/eLife.38493.003

Additional files

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https://doi.org/10.7554/eLife.38493.004

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