(A) Non-disjunction of the rDNA in cut14-208 cells. The nucleolar protein Gar2-mcherry was used as a marker for the rDNA (nucleolus) and the plasma membrane protein Psy1-GFP to visualise cytokinesis. Mutant cells and their isogenic wt controls were grown at 36°C for 2.5 hr (cut14-208 and ptr4-1) or 8 hr (mis6-302), fixed and stained with DAPI. Segregation of the rDNA in daughter nuclei was measured upon mitotic exit. Scale bar: 10 μm. (B) Rrp6 is enriched in the nucleolus, and depleted from anucleolate cut14-208 mutant cells. Indicated cells were grown at 36°C, fixed and processed for immunofluorescence against Gar2-GFP and Rrp6-myc. DNA was stained with DAPI. Lower panel shows the ratio of Rrp6-myc signals measured within daughter nuclei in septated cells. ***p<0.001, Wilcoxon ranked sum test with continuity correction. (C) Asymmetric partitioning of Dis3 in budding yeast upon condensin cleavage. pTEV protease expression was induced in cells synchronized in G1 phase by α-factor. 2.5 hr after TEV induction, cells were released from the arrest and segregation of Dis3-mNeonGreen was scored between 2 and 2.5 hr after the release by measuring the ratio between Dis3 signals in daughter to mother cells (N = 97 (strain C5259), N = 139 (strain C5260); box plot shows median (red) ±first quartile and 1.5x interquartile range (whiskers); ****p<0.0001, unpaired t-test with Welch’s correction). Scale bar: 5 μm. (D–E) The non-coding RNA mug93as accumulate in anucleolate cut14-208 cells. Cells of indicated genotype and expressing Gar2-GFP were grown at 36°C for 2.5 hr, fixed and processed for single molecule RNA FISH using probes complementary to the ncRNA mug93as (D) or the mRNA cct2 (E). Box and whiskers plots show quantifications of RNA spots in cut14-208 compared to wt, and in nucleolate compared to anucleolate mutant cells. ***p<0.001, *p<0.05 and °p>0.05, Wilcoxon non-parametric test.