Genetic reagent (Mus musculus) | KrasLSL-G12D | PMID: 11751630 | | Dr. Tyler Jacks (Massachusetts Institute of Technology, Cambridge, Massachusetts) |
Genetic reagent (M. musculus) | KrasFSF-G12D | PMID: 21512139 | RRID:MGI:5007794 | Dr. Tyler Jacks (Massachusetts Institute of Technology , Cambridge, Massachusetts) |
Genetic reagent (M. musculus) | Rosa26LSL-tdTomato | PMID: 20023653 | RRID:MGI:4436847 | Jackson Laboratories (Bar Harbor, Maine) |
Genetic reagent (M. musculus) | Rosa26FSF-CreERT2 | PMID: 25326799 | | Dr. Dieter Saur (Technische Universität München, München, Germany) |
Genetic reagent (M. musculus) | Nkx2-1F/F | PMID: 16601074 | RRID:MGI:3653706 | Dr. Shioko Kimura (National Cancer Institute (NCI), National Institutes of Health, Bethesda, Maryland) |
Genetic reagent (M. musculus)
| Foxa1F/F | PMID: 19141476 | RRID:MGI:3831163 | Dr. Klaus H. Kaestner (University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA) |
Genetic reagent (M. musculus) | Foxa2F/F | PMID: 10866673 | RRID:MGI:2177357 | Dr. Klaus H. Kaestner (University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA) |
Genetic reagent (M. musculus) | CAG-LSL-HA-UPRT | PMID: 23307870 | | Jackson Laboratories (Bar Harbor, Maine)
|
Cell line | 293T | PMID: 19561589 | | |
Antibody | Rat monoclonal anti-BrdU | Abcam | Cat. #: ab6326, RRID: AB_305426 | IHC (1:100) |
Antibody | Rabbit monoclonal anti-Cadherin 13 | Abcam | Cat. #: ab167407 | IHC (1:250) |
Antibody | Rabbit polyclonal anti-Cathepsin E | Lifespan Biosciences | Cat. #: LS-B523, RRID:AB_2087236 | IHC (1:12000) |
Antibody | Rabbit monoclonal anti-Caveolin 1 | Abcam | Cat. #: ab192869 | IHC (1:4000) |
Antibody | Rabbit monoclonal anti-CHIL3/4 | Abcam | Cat. #: ab192029 | IHC (1:20000) |
Antibody | Rabbit monoclonal anti-Cleaved- caspase 3 | Cell Signaling Technology | Cat. #: 9664 | IHC (1:800) |
Antibody | Rabbit monoclonal anti-Cytokeratin-5 | Abcam | Cat #: ab52635 (EP1691Y) | IHC (1:400) |
Antibody | Rabbit monoclonal anti-Cytokeratin-7 | Abcam | Cat #: ab181598 (EP17078) | IHC (1:20,000) |
Antibody | Rat monoclonal anti-Cytokeratin-8 | Developmental Studies Hybridoma Bank | Cat. #: TROMA-I, RRID:AB_531826 | IHC (1:100) |
Antibody | Rabbit monoclonal anti-Cytokeratin- 14 | Abcam | Cat. #: ab181595 (EPR17350) | IHC (1:4000) |
Antibody | Rabbit monoclonal anti-FoxA1 | Abcam | Cat. #: ab173287 | IHC (1:4000) |
Antibody | Rabbit monoclonal anti-FoxA2 | Abcam | Cat. #: ab108422, RRID:AB_11157157 | IHC (1:1200) |
Antibody | Goat polyclonal anti-Galectin 4 | R and D Systems | Cat. #: AF2128, RRID:AB_ 2297050 | IHC (1:400) |
Antibody | Mouse monoclonal anti-Gastrokine 1 | Abnova | Cat. #: H00056287-M01, RRID:AB_1505437 | IHC (1:50) |
Antibody | Rabbit monoclonal anti-GDA | Abcam | Cat. #: ab210606 | IHC (1:5000) |
Antibody | Rabbit monoclonal anti-HNF4α | Cell Signaling Technology | Cat. #: 3113S, RRID:AB_2295208 | IHC (1:500) |
Antibody | Rabbit monoclonal anti-Ki67 | Abcam | Cat. #: ab16667, RRID:AB_302459 | IHC (1:100) |
Antibody | Rabbit polyclonal anti-MCM2 | Abcam | Cat. #: ab31159, RRID:AB_881276 | IHC (1:800) |
Antibody | Polyclonal goat anti-MMP7 | R and D Systems | Cat. #: AF2967, RRID:AB_664120
| IHC (1:400) |
Antibody | Mouse monoclonal anti-Muc5AC | Abnova | Cat. #: MAB13117 | IHC (1:100) |
Antibody | Rabbit monoclonal anti-NKX2-1 | Abcam | Cat. #: ab76013, RRID: AB_1310784 | IHC (1:2000) |
Antibody | Mouse monoclonal anti-p40 (∆Np63) | Biocare Medical | Cat. #: ACI 3066 C | IHC (1:100) |
Antibody | Mouse monoclonal anti-PDX1 | Developmental Studies Hybridoma Bank | Cat. #: F109-D12, RRID:AB_1 157903 | IHC (1:10) |
Antibody | Rat monoclonal anti-PIGR | Abcam | Cat. #: ab170321 | IHC (1:400) |
Antibody | Rabbit monoclonal anti-PK-LR | Abcam | Cat. #: ab171744 | IHC (1:500) |
Antibody | Rabbit polyclonal anti-proSPC | Millipore | Cat. #: AB3786, RRID: AB_91588 | IHC (1:4000) |
Antibody | Rabbit polyclonal anti-RFP | Rockland | Cat. #: 600-401-379 | IHC (1:400) |
Antibody | Rabbit monoclonal anti-SOX2 | Cell Signaling Technology | Cat. #: 3728, RRID: AB_2194037 | IHC (1:250) |
Antibody | Rabbit monoclonal anti-VCAM1 | Abcam | Cat. #: ab134047, RRID:AB_2721053 | IHC (1:1000) |
Recombinant DNA reagent | Ad5-CMVCre | Gene Transfer Vector Core, University of Iowa, IA | VVC-U of Iowa-5-HT | |
Recombinant DNA reagent | Ad5-CMVFlpo | Gene Transfer Vector Core, University of Iowa, IA | VVC-U of Iowa-530HT | |
Recombinant DNA reagent | Ad5-SPC-Cre | Gene Transfer Vector Core, University of Iowa, IA | VVC-Berns- 1168 | |
Recombinant DNA reagent | Ad5-SPC-FlpO | Gene Transfer Vector Core, University of Iowa, IA | VVC-Snyder- 6695 | |
Recombinant DNA reagent | PGK-Cre | PMID: 19561589 | | |
Recombinant DNA reagent | VSVg | PMID: 19561589 | | |
Recombinant DNA reagent | Δ8.9 | PMID: 19561589 | | |
Recombinant DNA reagent | SPC-FlpO shuttle plasmid | this paper | | |
Chemical compound, drug | Tamoxifen | Sigma-Aldrich | T5648-5G | |
Chemical compound, drug | Tamoxifen supplemented chow | Envigo | TD.130858 | 500 mg/kg of diet |
Chemical compound, drug | 1X PBS | ThermoFisher Scientific | 20012050 | |
Chemical compound, drug | Trizol | ThermoFisher Scientific | 15596026 | |
Commercial assay, kit | Bloxall | Vector Laboratories | SP-6000 | |
Commercial assay, kit | Horse serum | Vector Laboratories | S-2012 | |
commercial assay, kit | Rodent Block M | Biocare Medical | RBM961 | |
Commercial assay, kit | ImmPRESS anti-rabbit HRP | Vector Laboratories | MP-7401 | |
Commercial assay, kit | ImmPRESS anti-rat HRP | Vector Laboratories | MP-7444 | |
Commercial assay, kit | ImmPRESS anti-goat HRP | Vector Laboratories | MP-7405 | |
Commercial assay, kit | Anti-mouse secondary | Biocare Medical | MM620 | |
Commercial assay, kit | ImmPACT DAB Peroxidase (HRP) Substrate | Vector Laboratories | SK-4105 | |
Commercial assay, kit | ImmPACT VIP Peroxidase (HRP) Substrate | Vector Laboratories | SK-4605 | |
Commercial assay, kit | Hematoxylin | Fisher Scientific | 6765003 | |
Commercial assay, kit | Collagena se type I | ThermoFisher Scientific | 17100017 | |
Commercial assay, kit | Elastase | Worthington Biochemical Corporation | LS002280 | |
Commercial assay, kit | Dispase | Corning | 354235 | |
Commercial assay, kit | Deoxyribonuclease I | Sigma-Aldrich | DN25 | |
Commercial assay, kit | Red Blood Cell Lysis Buffer | eBioscience | 00-4333-57 | |
Commercial assay, kit | PureLink RNA Mini kit | ThermoFisher Scientific | 12183018A | |