Multiple neurons encode CrebB dependent appetitive long-term memory in the mushroom body circuit
- University of Fribourg, Switzerland
Abstract
Lasting changes in gene expression are critical for the formation of long-term memories (LTMs), depending on the conserved CrebB transcriptional activator. While requirement of distinct neurons in defined circuits for different learning and memory phases have been studied in detail, only little is known regarding the gene regulatory changes that occur within these neurons. We here use the fruit fly as powerful model system to study the neural circuits of CrebB-dependent appetitive olfactory LTM. We edited the CrebB locus to create a GFP-tagged CrebB conditional knockout allele, allowing us to generate mutant, post-mitotic neurons with high spatial and temporal precision. Investigating CrebB-dependence within the mushroom body (MB) circuit we show that MB α/β and α'/β' neurons as well as MBON α3, but not in dopaminergic neurons require CrebB for LTM. Thus, transcriptional memory traces occur in different neurons within the same neural circuit.
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All data is included in the manuscript.
Article and author information
Author details
Funding
Bundesbehörden der Schweizerischen Eidgenossenschaft (SynaptiX)
- Simon G Sprecher
Novartis Stiftung für Medizinisch-Biologische Forschung (18A017)
- Simon G Sprecher
Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (CRSII5_180316)
- Simon G Sprecher
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Leslie C Griffith, Brandeis University, United States
Publication history
- Received: June 13, 2018
- Accepted: October 19, 2018
- Accepted Manuscript published: October 22, 2018 (version 1)
- Version of Record published: November 13, 2018 (version 2)
Copyright
© 2018, Widmer et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Multiple neurons encode CrebB dependent appetitive long-term memory
in the mushroom body circuit
eLife 7:e39196.
https://doi.org/10.7554/eLife.39196
