Multiple neurons encode CrebB dependent appetitive long-term memory in the mushroom body circuit

Abstract

Lasting changes in gene expression are critical for the formation of long-term memories (LTMs), depending on the conserved CrebB transcriptional activator. While requirement of distinct neurons in defined circuits for different learning and memory phases have been studied in detail, only little is known regarding the gene regulatory changes that occur within these neurons. We here use the fruit fly as powerful model system to study the neural circuits of CrebB-dependent appetitive olfactory LTM. We edited the CrebB locus to create a GFP-tagged CrebB conditional knockout allele, allowing us to generate mutant, post-mitotic neurons with high spatial and temporal precision. Investigating CrebB-dependence within the mushroom body (MB) circuit we show that MB α/β and α'/β' neurons as well as MBON α3, but not in dopaminergic neurons require CrebB for LTM. Thus, transcriptional memory traces occur in different neurons within the same neural circuit.

Data availability

All data is included in the manuscript.

Article and author information

Author details

  1. Yves F Widmer

    Department of Biology, University of Fribourg, Fribourg, Switzerland
    Competing interests
    The authors declare that no competing interests exist.
  2. Cornelia Fritsch

    Department of Biology, University of Fribourg, Fribourg, Switzerland
    Competing interests
    The authors declare that no competing interests exist.
  3. Magali M Jungo

    Department of Biology, University of Fribourg, Fribourg, Switzerland
    Competing interests
    The authors declare that no competing interests exist.
  4. Silvia Almeida

    Department of Biology, University of Fribourg, Fribourg, Switzerland
    Competing interests
    The authors declare that no competing interests exist.
  5. Boris Egger

    Department of Biology, University of Fribourg, Fribourg, Switzerland
    Competing interests
    The authors declare that no competing interests exist.
  6. Simon G Sprecher

    Department of Biology, University of Fribourg, Fribourg, Switzerland
    For correspondence
    simon.sprecher@unifr.ch
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-9060-3750

Funding

Bundesbehörden der Schweizerischen Eidgenossenschaft (SynaptiX)

  • Simon G Sprecher

Novartis Stiftung für Medizinisch-Biologische Forschung (18A017)

  • Simon G Sprecher

Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (CRSII5_180316)

  • Simon G Sprecher

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Leslie C Griffith, Brandeis University, United States

Publication history

  1. Received: June 13, 2018
  2. Accepted: October 19, 2018
  3. Accepted Manuscript published: October 22, 2018 (version 1)
  4. Version of Record published: November 13, 2018 (version 2)

Copyright

© 2018, Widmer et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

Metrics

  • 1,701
    Page views
  • 264
    Downloads
  • 11
    Citations

Article citation count generated by polling the highest count across the following sources: PubMed Central, Crossref, Scopus.

Download links

A two-part list of links to download the article, or parts of the article, in various formats.

Downloads (link to download the article as PDF)

Open citations (links to open the citations from this article in various online reference manager services)

Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)

  1. Yves F Widmer
  2. Cornelia Fritsch
  3. Magali M Jungo
  4. Silvia Almeida
  5. Boris Egger
  6. Simon G Sprecher
(2018)
Multiple neurons encode CrebB dependent appetitive long-term memory in the mushroom body circuit
eLife 7:e39196.
https://doi.org/10.7554/eLife.39196