Myosin V functions as a vesicle tether at the plasma membrane to control neurotransmitter release in central synapses
- Washington University, United States
Abstract
Synaptic vesicle fusion occurs at specialized release sites at the active zone. How refilling of release sites with new vesicles is regulated in central synapses remains poorly understood. Using nanoscale-resolution detection of individual release events in rat hippocampal synapses we found that inhibition of myosin V, the predominant vesicle-associated motor, strongly reduced refilling of the release sites during repetitive stimulation. Single-vesicle tracking revealed that recycling vesicles continuously shuttle between a plasma membrane pool and an inner pool. Vesicle retention at the membrane pool was regulated by neural activity in a myosin V dependent manner. Ultrastructural measurements of vesicle occupancy at the plasma membrane together with analyses of single-vesicle trajectories during vesicle shuttling between the pools suggest that myosin V acts as a vesicle tether at the plasma membrane, rather than a motor transporting vesicles to the release sites, or directly regulating vesicle exocytosis.
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All data generated or analyzed during this study are included in the manuscript and supporting information provided.
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Funding
National Institute of Neurological Disorders and Stroke (NS105776)
- Vitaly Klyachko
CIMED Center at Washington University
- Vitaly Klyachko
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Ethics
Animal experimentation: All animal procedures were in compliance with the US National Institutes of Health Guide for the Care and Use of Laboratory Animals. All animal procedures conformed to the guidelines approved by the Washington University Animal Studies Committee (protocol approval # 20170233).
Copyright
© 2018, Maschi et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Myosin V functions as a vesicle tether at the plasma membrane to control neurotransmitter release in central synapses
eLife 7:e39440.
https://doi.org/10.7554/eLife.39440
