(A) Whole abdomen of a virgin female showing the location of the mating system. T8 and 9 indicate tergites 8 and 9. (B) Light microscopy photograph of the spermathecal plate with a single seminal capsule. (C) CLSM image of the spermathecal plate. (D) 3D segmentation of the spermathecal plate with no seminal capsule. Dotted line indicates the section plane shown in F and G. (E) 3D segmentation of the spermathecal plate with two seminal capsules and showing seminal flow. (F) Attachment points for the seminal capsules in the virgin female. (G) The base of two seminal capsules in different stages of connection to the spermathecal plate (corresponding to the two seminal capsules in Figure 1E).